Overview

  • Product name

    Anti-AE2 antibody - N-terminal
    See all AE2 primary antibodies
  • Description

    Rabbit polyclonal to AE2 - N-terminal
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Monkey, Common marmoset, Orangutan, Elephant
  • Immunogen

    Synthetic 18 amino acid peptide from the N terminus of Human AE2.

  • Positive control

    • Human kidney and breast tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab140953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.

References

ab140953 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.25% Triton-X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 01 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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