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    ae2-antibody-n-terminal-ab140953.pdf

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Anti-AE2 antibody - N-terminal (ab140953)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)

Key features and details

  • Rabbit polyclonal to AE2 - N-terminal
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-AE2 antibody - N-terminal
    See all AE2 primary antibodies
  • Description

    Rabbit polyclonal to AE2 - N-terminal
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Monkey, Common marmoset, Orangutan, Elephant
  • Immunogen

    Synthetic 18 amino acid peptide from the N terminus of Human AE2.

  • Positive control

    • Human kidney and breast tissues
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab140953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plasma membrane anion exchange protein of wide distribution.
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P04920 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 463890 Chimpanzee
    • Entrez Gene: 100410591 Common marmoset
    • Entrez Gene: 404084 Cow
    • Entrez Gene: 475538 Dog
    • Entrez Gene: 791243 Horse
    • Entrez Gene: 6522 Human
    • Entrez Gene: 20535 Mouse
    • Entrez Gene: 100172030 Orangutan
    • Entrez Gene: 397203 Pig
    • Entrez Gene: 100009159 Rabbit
    • Entrez Gene: 24780 Rat
    • Omim: 109280 Human
    • SwissProt: Q6SJP2 Horse
    • SwissProt: P04920 Human
    • SwissProt: P13808 Mouse
    • SwissProt: Q5RD44 Orangutan
    • SwissProt: P48746 Rabbit
    • SwissProt: P23347 Rat
    • Unigene: 647069 Human
    • Unigene: 4580 Mouse
    • Unigene: 9860 Rat
    see all
  • Alternative names

    • AE 2 antibody
    • AE2 anion exchanger antibody
    • Anion exchange protein 2 antibody
    • Anion exchanger 2 antibody
    • B3A2_HUMAN antibody
    • BAND 3-LIKE PROTEIN antibody
    • BND3L antibody
    • EPB3L1 antibody
    • erythrocyte band 3 antibody
    • Erythrocyte membrane protein band 3 like 1 antibody
    • HKB3 antibody
    • MPB3L antibody
    • NBND3 antibody
    • Non-erythroid band 3-like protein antibody
    • Slc4a2 antibody
    • Solute carrier family 4 member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AE2 antibody - N-terminal (ab140953)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labelling AE2 with ab140953 at 10 µg/ml after heat-induced antigen retrieval.

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab140953? Please let us know so that we can cite the reference in this datasheet.

    ab140953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-AE2 antibody - N-terminal

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.25% Triton-X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 01 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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