Immunohistochemistry (Frozen sections) abreview for Anti-AE2 antibody - N-terminal

Immunohistochemistry (Frozen sections)

Mouse Tissue sections (Brain)

Yes - 0.25% Triton-X

Brain

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Paraformaldehyde

1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: Blocking Buffer

1/20

1/1000

Non-Abcam antibody was used: Thremo Fisher anti-Rabbit IgG-488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

1/20, 1/200, and 1/2000 dilution of the primary ab did not show anything other than background staining.

At the time of this Abreview, we have not yet tested or validated product Anti-AE2 antibody - N-terminal (ab140953) for use in IHC-Fr applications with mouse samples. With further optimization, it is possible that this Ab can be successful in this combination of sample species and application. Our Scientific Support Team is currently investigating this Abreview to determine how best to optimize this experiment.