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Mouse monoclonal [2012C4a] to AEBP2
Recombinant fragment from the N-terminal of human AEBP2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Protein G purified
ab74517 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
Use at an assay dependent dilution.
DNA-binding transcriptional repressor. May interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
Belongs to the AEBP2/jing C2H2-type zinc-finger family.
Contains 3 C2H2-type zinc fingers.
Information by UniProt
Adipocyte enhancer binding protein 2 antibody
Adipocyte enhancer-binding protein 2 antibody
AE (adipocyte enhancer) binding protein 2 antibody
Western blot - Anti-AEBP2 antibody [2012C4a] (ab74517)
Anti-AEBP2 antibody [2012C4a] (ab74517) + Immunising recombinant protein
Predicted band size:
Observed band size:
35 kDa (
why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"