Overview

  • Product name

    AEC (3-Amino-9-ethylcarbazole), Peroxidase substrate
  • Description

    Peroxidase substrate
  • Alternative names

    • 3-Amino-9-ethylcarbazole
  • Biological description

    Peroxidase substrate. Insoluble, red chromogen used for antibody detection in immunohistochemistry supplied as a 50X concentrate (0.095 mol/l).


     

  • CAS Number

    132-32-1
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    9-Ethylcarbazol-3-amine
  • Molecular weight

    210.27
  • Molecular formula

    C14H14N2
  • PubChem identifier

    8588
  • Storage instructions

    Store at +4°C. The product can be stored for up to 12 months.
  • Solubility overview

    Supplied in DMF (2%)
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Nakata T & Suzuki N Chromogen-based immunohistochemical method for elucidation of the coexpression of two antigens using antibodies from the same species. J Histochem Cytochem 60:611-9 (2012). Read more (PubMed: 22610462) »
  • Zehntner SP  et al. Synergistic tissue counterstaining and image segmentation techniques for accurate, quantitative immunohistochemistry. J Histochem Cytochem 56:873-80 (2008). Read more (PubMed: 18574255) »
  • Krieg R  et al. Novel chromogenic substrates with metal chelating properties for the histochemical detection of peroxidasic activity, derived from 3-amino-9-ethylcarbazole (AEC) and 3,6-diamino-9-ethylcarbazole. Cell Mol Biol (Noisy-le-grand) 46:1191-212 (2000). Read more (PubMed: 11075949) »
See 1 Publication for this product

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