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    aec-singleplus-30ml-ab103742.pdf

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AEC Single/Plus (30ml) (ab103742)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)

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Overview

  • Product name

    AEC Single/Plus (30ml)
  • General notes

    AEC Single/Plus is a single, highly stable AEC chromogen/substrate working solution. When used in conjugation with immunoperoxidase detection systems, AEC produces a red colored and product at positive sites, yeilding strong contrast when combined with a blue hematoxylin counterstain. Specimens stained using AEC Single/Plus cannot be dehydrated in ethanol and must be mounted in an aqueous-based mounting medium.

    AEC Single/Plus (ab103742) is a single, stable ready for use solution. It can be used up until the expiry date so reduces waste when compared to DAB substrate/chromgen solutions. The solution is light sensitive and must be protected from exposure to light and stored in an opaque bottle or dark environment.

    Protocol

    • Once section have been incubated with peroxidase, wash with wash buffer.
    • Wipe slides removing excess buffer. Add enough drops of AEC Single/Plus to cover tissue sections.
    • Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under the microscope for signal developmant. Once the desired signal to noise ratio is acheived, stop the reaction by washing the slides in DI H2O.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at +4°C. Store In the Dark.
  • Storage buffer

    Constituent: 6% Hydrogen peroxide
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
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    • Substrate

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
    AEC Single/Plus staining Cytokeratin 5 & 14 in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Single/Plus (30ml) (ab103742)
    Formalin fixed paraffin embedded Human tonsil stained with HMW Cytokeratin antibody (ab850) labeled with AEC Single/Plus (30ml) (ab103742) produces a robust red color.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab103742? Please let us know so that we can cite the reference in this datasheet.

ab103742 has been referenced in 1 publication.

  • Kroth H  et al. Discovery and preclinical characterization of [18F]PI-2620, a next-generation tau PET tracer for the assessment of tau pathology in Alzheimer's disease and other tauopathies. Eur J Nucl Med Mol Imaging 46:2178-2189 (2019). PubMed: 31264169

Customer reviews and Q&As

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Question

Hello,I want to buy substrats for Alkaline phosphatase and HRP for double elispot test.I have seen that you have for alkaline phosphatase  BCIP /NBT :ab7413ab7468for HRP AEC :ab103742ab64252but it is only mentionned for IHC.Can I use these reagents for elispot?Do they need to be filtered or not?What is the protocol (volume to dispense, dilution...?)Thanks for your answer.

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Abcam community

Verified customer

Asked on Sep 08 2011

Answer

Thank you very much for your enquiry. We do not currently sell substrates such as AP and HRP that have been tested in ELISPOT. This doesn’t mean that these products are not suitable, we just have not tested them in this application. The 4 products identified (ab7413, ab7468, ab64252, ab103742) have been tested in IHC and ab7413 has also been tested in dot blots and WB. This may be a more suitable one to try but unfortunately we cannot guarantee that it would be suitable. I would consider reviewing what ELISPOT kits we have available as we may have one that is suitable. I would be happy to offer any further advice and hope this information helps.

Read More

Abcam Scientific Support

Answered on Sep 08 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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