Product nameAEC Substrate Kit
DescriptionAEC Substrate Kit
Tested applicationsSuitable for: IHC-Pmore details
AEC substrate kit for use in IHC using HRP / peroxidase detection. Ready to use formulation.
Previously called AEC Substrate System (Ready to Use).
IHC protocol suitable for use with AEC Substrate Kit ab64252:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
5. Apply primary antibody in antibody diluent and incubate.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC Substrate ab64252 and incubate until desired color is achieved (1-10 mins, recommended 10 mins for AEC). Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Storage instructionsStore at +4°C.
Storage bufferConstituents: 0.03% Hydrogen peroxide, 3% DMF
Concentration information loading...
RelevanceAEC (3-Amino-9-Ethylcarbazole) is a widely used chromogen for immunohistochemical staining. AEC produces a red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. AEC produces a clean, very intense stain that is free of unwanted background.
- 3 amino 9 ethylcarbazole
Our Abpromise guarantee covers the use of ab64252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
This product has been referenced in:
- Maliniemi P et al. Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas. Oncoimmunology 6:e1273310 (2017). Read more (PubMed: 28405495) »
- Brachelente C et al. Transcriptome Analysis of Canine Cutaneous Melanoma and Melanocytoma Reveals a Modulation of Genes Regulating Extracellular Matrix Metabolism and Cell Cycle. Sci Rep 7:6386 (2017). Read more (PubMed: 28743863) »