Recombinant Anti-AER61 antibody [EPR12944] (ab190693)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12944] to AER61
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-AER61 antibody [EPR12944]
See all AER61 primary antibodies -
Description
Rabbit monoclonal [EPR12944] to AER61 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Neuro-2a, U87-MG, HeLa, Caco 2, C6, Raw264.7 cell lysates; Mouse brain, heart, kidney and spleen, Rat brain, heart, kidney and spleen tissue lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12944 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- RAW 264.7 whole cell lysate (ab7187)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
Target
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Relevance
Function: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains. Disease: Adams-Oliver syndrome 4 Similarity: Belongs to the glycosyltransferase 61 family. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 285203 Human
- Entrez Gene: 101351 Mouse
- Entrez Gene: 494219 Rat
- Omim: 614789 Human
- SwissProt: Q5NDL2 Human
- SwissProt: Q8BYW9 Mouse
- SwissProt: Q5NDL0 Rat
- Unigene: 518059 Human
see all -
Alternative names
- AER61 glycosyltransferase antibody
- AOS4 antibody
- C3orf64 antibody
see all
Images
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All lanes : Anti-AER61 antibody [EPR12944] (ab190693) at 1/1000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa -
All lanes : Anti-AER61 antibody [EPR12944] (ab190693) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lane 9 : C6 cell lysate
Lane 10 : Raw264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated
Predicted band size: 62 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab190693 has been referenced in 1 publication.
- Barua R et al. Bioinformatics and Functional Analyses Implicate Potential Roles for EOGT and L-fringe in Pancreatic Cancers. Molecules 26:N/A (2021). PubMed: 33562410