Western blot abreview for Anti-AF10 antibody [HAF10 10D3/8]

Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3-L1 differentiated adipocytes)
Loading amount
50 µg
Specification
3T3-L1 differentiated adipocytes
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse IgG goat Perkin Elmer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Positive control
Same cell extracts treated with anti-actin (MAB1501R) 1:2000 and anti-H2B (ab1790) 1:2000 antibodies.
anti-mouse and anti-rabbit secondary HRP conjugated antibodies 1:10000.

Additional data

Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Dr. Ainhoa Ceballos

Verified customer

Submitted Apr 10 2013

Sign up