Western blot abreview for Anti-AF9 antibody

Average
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (primary bone marrow derived macrophages)
Specification
primary bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit HRP biorad
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Clarity ECL biorad
Bands
Specific: 65 kDa
Exposure
3 minute(s) and 0 second(s)

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Submitted Sep 05 2014

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