• Product name
  • Description
    Rabbit polyclonal to AF9
  • Host species
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ASSCAVQVKL ELGHRAQVRK KPTVEGFTHD WMVFVRGPEH SNIQHFVEKV) of human AF9 (NP_004520)

  • Positive control
    • HepG2 cell lysate IF/ICC: HepG2 cell line.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab80558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

ICC/IF Use a concentration of 10 µg/ml.


  • Involvement in disease
    Note=A chromosomal aberration involving MLLT3 is associated with acute leukemias. Translocation t(9;11)(p22;q23) with MLL/HRX. The result is a rogue activator protein.
  • Sequence similarities
    Contains 1 YEATS domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • AF 9 antibody
    • AF9_HUMAN antibody
    • ALL1 fused gene from chromosome 9 protein antibody
    • ALL1-fused gene from chromosome 9 protein antibody
    • FLJ2035 antibody
    • MLLT3 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein antibody
    • Protein AF-9 antibody
    • YEATS 3 antibody
    • YEATS domain containing protein 3 antibody
    • YEATS domain-containing protein 3 antibody
    • YEATS3 antibody
    see all


  • Western blot analysis of HeLa cell lysate labeling AF9 with ab80558 at 1.0µg/ml.
  • ICC/IF image of ab80558 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80558, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-AF9 antibody (ab80558) at 1 µg/ml + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa

    Gel concentration: 12%


ab80558 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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Thank you for your reply and for providing these details of your protocol. I agree that the background bands you detected should not be present using your protocol. The only suggestion I could make is that because AF9 is a nuclear protein, you may see ...

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Thank you for contacting us. I am sorry that this antibody is detecting background bands and weak signal in your samples. Could you please provide a few additional details of your protocol?

1) Was the transfected AF9 also human?

2) ...

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