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Rabbit polyclonal to AFAF
antigen: SCESQYSVNP ELATMSYFHP SEGVSDTSFS KSAESSTFLG TTSSDMRRSG TRTSESKIMT DIISIGSDNE MHENDESVTR, corresponding to amino acids 215-294 of Human AFAF
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Involved in acrosome biogenesis.
Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
Cytoplasmic vesicle > secretory vesicle > acrosome membrane.
Information by UniProt
Acrosome formation-associated factor antibody
Immunocytochemistry/ Immunofluorescence - Anti-AFAF antibody (ab121461)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121461 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFAF antibody (ab121461)
ab121461 at a 1/300 dilution staining AFAF in Human testis tissue (paraffin embedded) by immunohistochemistry.
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