Anti-AFAF/Equatorin antibody (ab121470)
Key features and details
- Rabbit polyclonal to AFAF/Equatorin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AFAF/Equatorin antibody
See all AFAF/Equatorin primary antibodies -
Description
Rabbit polyclonal to AFAF/Equatorin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human AFAF/Equatorin aa 35-182.
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Positive control
- Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in acrosome biogenesis. -
Tissue specificity
Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > acrosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 54586 Human
- SwissProt: Q9NQ60 Human
- Unigene: 163070 Human
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Alternative names
- Acrosome formation-associated factor antibody
- Afaf antibody
- AFAF_HUMAN antibody
see all
Images
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Lanes 2-3 : Anti-AFAF/Equatorin antibody (ab121470)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing AFAF/Equatorin with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AFAF/Equatorin with ab121470. Staining shows strong cytoplasmic positivity in spermatids.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121470 has not yet been referenced specifically in any publications.