Overview

  • Product name

    Anti-AFAF/Equatorin antibody
    See all AFAF/Equatorin primary antibodies
  • Description

    Rabbit polyclonal to AFAF/Equatorin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human AFAF/Equatorin aa 35-182.

  • Positive control

    • Human testis tissue.
  • General notes

     This product was previously labelled as AFAF

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in acrosome biogenesis.
  • Tissue specificity

    Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle > acrosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acrosome formation-associated factor antibody
    • Afaf antibody
    • AFAF_HUMAN antibody
    • C9orf11 antibody
    • chromosome 9 open reading frame 11 antibody
    • equatorin antibody
    see all

Images

  • Lanes 2-3 : Anti-AFAF/Equatorin antibody (ab121470)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing AFAF/Equatorin with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AFAF/Equatorin with ab121470. Staining shows strong cytoplasmic positivity in spermatids.

References

ab121470 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121470.
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