Anti-AFF4 antibody (ab220039)

Submit a review Submit a question

Overview

  • Product name

    Anti-AFF4 antibody
    See all AFF4 primary antibodies

  • Description

    Rabbit polyclonal to AFF4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment corresponding to Human AFF4 aa 56-199.
    Sequence:

    LGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQS SKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRES YNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHG


    Database link: Q9UHB7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: U-251 MG cell lysate. ICC/IF: CACO-2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab220039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 127 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Relevance

    May play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • AF4/FMR2 family member 4 antibody
      • AF5Q31 antibody
      • Alf4 antibody
      • ALL1 fused gene from chromosome 5q31 antibody
      • CHOPS antibody
      • HSPC092 antibody
      • Laf4 antibody
      • Lymphoid nuclear protein related to AF4 like antibody
      • Major CDK9 elongation factor associated protein antibody
      • MCEF antibody
      • MGC107469 antibody
      • RP23-226J16.7 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-AFF4 antibody (ab220039)
      Immunocytochemistry/ Immunofluorescence - Anti-AFF4 antibody (ab220039)

      Immunofluorescent analysis of formalin fixed, triton X-100 permeabilized CACO-2 cells labeling AFF4 using ab220039 at 4 μg/ml (green).

    • Western blot - Anti-AFF4 antibody (ab220039)
      Western blot - Anti-AFF4 antibody (ab220039)
      All lanes : Anti-AFF4 antibody (ab220039) at 1/100 dilution

      Lane 1 : RT-4 cell lysate
      Lane 2 : U-251 MG cell lysate

      Predicted band size: 127 kDa

    References

    ab220039 has not yet been referenced specifically in any publications.

    Publishing research using ab220039? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab220039.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com