Key features and details
- Rabbit polyclonal to AFF4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AFF4 antibody
See all AFF4 primary antibodies
DescriptionRabbit polyclonal to AFF4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Non human primates
Synthetic peptide corresponding to Human AFF4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following mouse tissue lysates: Liver; Hippocampus; and cell lysate U87-MG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).|
RelevanceMay play a role in transcriptional regulation. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb).
- AF4/FMR2 family member 4 antibody
- AF5Q31 antibody
- Alf4 antibody
All lanes : Anti-AFF4 antibody (ab75866) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 127 kDa
Observed band size: 127 kDa
Additional bands at: 109 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
ab75866 has not yet been referenced specifically in any publications.