Key features and details
- Rabbit polyclonal to AFG2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AFG2 antibody
See all AFG2 primary antibodies
DescriptionRabbit polyclonal to AFG2
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human AFG2 aa 200-250. The exact sequence is proprietary.
Database link: Q8NB90
- IP: HeLa cell lysate.
This product was previously labelled as SPATA5
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceSPATA5 may be involved in morphological and functional mitochondrial transformations during spermatogenesis.
Cellular localizationCytoplasmic and Mitochondrial
- AFG2 antibody
- ATPase family gene 2 homolog antibody
- ATPase family protein 2 homolog antibody
AFG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241342 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241342 at 1 µg/ml.
Lane 1: ab241342 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241342 has not yet been referenced specifically in any publications.