Overview

  • Product name

  • Description

    Rabbit polyclonal to AFG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AFG2 aa 843-893. The exact sequence is proprietary.
    Database link: Q8NB90

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    ab241369 has not performed satisfactorily when used for WB of AFG2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g.immunoprecipitated) sources of AFG2.

     This product was previously labelled as SPATA5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab241369 was affinity purified using an epitope specific to AFG2 immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      SPATA5 may be involved in morphological and functional mitochondrial transformations during spermatogenesis.
    • Cellular localization

      Cytoplasmic and Mitochondrial
    • Database links

    • Alternative names

      • AFG2 antibody
      • ATPase family gene 2 homolog antibody
      • ATPase family protein 2 homolog antibody
      • SPAF antibody
      • Spermatogenesis associated 5 antibody
      • Spermatogenesis associated factor SPAF antibody
      • Spermatogenesis-associated factor protein antibody
      • spermatogenesis-associated protein 5 antibody
      see all

    Images

    • AFG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, cells prepared using NETN lysis buffer) using ab241369 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241369 at 1 µg/ml.

      Lane 1: ab241369 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

       

    References

    ab241369 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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