Key features and details
- Rabbit polyclonal to AFG2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AFG2 antibody
See all AFG2 primary antibodies
DescriptionRabbit polyclonal to AFG2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human AFG2 aa 843-893. The exact sequence is proprietary.
Database link: Q8NB90
- IP: HeLa whole cell lysate.
ab241369 has not performed satisfactorily when used for WB of AFG2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g.immunoprecipitated) sources of AFG2.
This product was previously labelled as SPATA5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241369 was affinity purified using an epitope specific to AFG2 immobilized on solid support
Our Abpromise guarantee covers the use of ab241369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceSPATA5 may be involved in morphological and functional mitochondrial transformations during spermatogenesis.
Cellular localizationCytoplasmic and Mitochondrial
- AFG2 antibody
- ATPase family gene 2 homolog antibody
- ATPase family protein 2 homolog antibody
AFG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, cells prepared using NETN lysis buffer) using ab241369 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241369 at 1 µg/ml.
Lane 1: ab241369 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241369 has not yet been referenced specifically in any publications.