Overview

  • Product name

    Anti-AFG2 antibody [EPR14038]
    See all AFG2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14038] to AFG2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human AFG2 aa 200-350. The exact sequence is proprietary.
    Database link: Q8NB90

  • Positive control

    • 293, Jurkat and A431 cell lysates; Human testis lysate; Jurkat cells.
  • General notes

     

     

     This product was previously labelled as SPATA5

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    SPATA5 may be involved in morphological and functional mitochondrial transformations during spermatogenesis.
  • Cellular localization

    Cytoplasmic and Mitochondrial
  • Database links

  • Alternative names

    • AFG2 antibody
    • ATPase family gene 2 homolog antibody
    • ATPase family protein 2 homolog antibody
    • SPAF antibody
    • Spermatogenesis associated 5 antibody
    • Spermatogenesis associated factor SPAF antibody
    • Spermatogenesis-associated factor protein antibody
    • spermatogenesis-associated protein 5 antibody
    see all

Images

  • All lanes : Anti-AFG2 antibody [EPR14038] (ab189519) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • All lanes : Anti-AFG2 antibody [EPR14038] (ab189519) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling AFG2 with ab189519 at 1/80 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab189519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189519.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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