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Signal Transduction Metabolism Mitochondrial
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Anti-AFG3L2 antibody (ab154990)

  • Datasheet
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Western blot - Anti-AFG3L2 antibody (ab154990)
  • Immunocytochemistry/ Immunofluorescence - Anti-AFG3L2 antibody (ab154990)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFG3L2 antibody (ab154990)

Key features and details

  • Rabbit polyclonal to AFG3L2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-AFG3L2 antibody
    See all AFG3L2 primary antibodies
  • Description

    Rabbit polyclonal to AFG3L2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Sheep, Rabbit, Cow, Cat, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse AFG3L2 aa 50-150 conjugated to keyhole limpet haemocyanin.
    Database link: Q8JZQ2

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Hippocampus; Rat Hippocampus; Mouse Cerebellum. This antibody gave a positive result in IHC in the following FFPE tissue: Normal mouse brain. This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    ATP-dependent protease which is essential for axonal development.
  • Tissue specificity

    Ubiquitous. Highly expressed in the cerebellar Purkinje cells.
  • Involvement in disease

    Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) [MIM:610246]. It is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA28 is an autosomal dominant cerebellar ataxia (ADCA) with a slow progressive course and no evidence of sensory involvement or cognitive impairment.
    Defects in AFG3L2 are the cause of spastic ataxia autosomal recessive type 5 (SPAX5) [MIM:614487]. A neurodegenerative disorder characterized by early onset spasticity, peripheral neuropathy, ptosis, oculomotor apraxia, dystonia, cerebellar atrophy, and progressive myoclonic epilepsy.
  • Sequence similarities

    In the N-terminal section; belongs to the AAA ATPase family.
    In the C-terminal section; belongs to the peptidase M41 family.
  • Cellular localization

    Mitochondrion membrane.
  • Target information above from: UniProt accession Q9Y4W6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 515757 Cow
    • Entrez Gene: 69597 Mouse
    • Entrez Gene: 307350 Rat
    • SwissProt: Q2KJI7 Cow
    • SwissProt: Q8JZQ2 Mouse
    • Unigene: 426052 Mouse
    • Unigene: 8386 Rat
    • Alternative names

      • AFG3 (ATPase family gene 3, yeast) like 2 antibody
      • AFG3 ATPase family gene 3 like 2 (yeast) antibody
      • AFG3 ATPase family gene 3 like 2 antibody
      • AFG3 like protein 2 antibody
      • AFG3-like protein 2 antibody
      • AFG32_HUMAN antibody
      • AFG3L2 antibody
      • ATPase family gene 3 like 2 antibody
      • ATPase family gene 3 yeast antibody
      • EC 3.4.24.- antibody
      • FLJ25993 antibody
      • Paraplegin like protein antibody
      • Paraplegin-like protein antibody
      • SCA28 antibody
      • Spinocerebellar ataxia 28 antibody
      see all

    Images

    • Western blot - Anti-AFG3L2 antibody (ab154990)
      Western blot - Anti-AFG3L2 antibody (ab154990)
      All lanes : Anti-AFG3L2 antibody (ab154990) at 1 µg/ml (Milk blocking 3%)

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Cerebellum Rat Tissue Lysate
      Lane 4 : Mouse Hippocampus Tissue Lysate
      Lane 5 : Rat Hippocampus Tissue Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 89 kDa
      Observed band size: 89 kDa
      Additional bands at: 68 kDa (possible non-specific binding)


      Exposure time: 2 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab154990 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    • Immunocytochemistry/ Immunofluorescence - Anti-AFG3L2 antibody (ab154990)
      Immunocytochemistry/ Immunofluorescence - Anti-AFG3L2 antibody (ab154990)

      ICC/IF image of ab154990 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab154990 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFG3L2 antibody (ab154990)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFG3L2 antibody (ab154990)

      IHC image of AFG3L2 staining in Normal mouse brain formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154990, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154990? Please let us know so that we can cite the reference in this datasheet.

    ab154990 has not yet been referenced specifically in any publications.

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