• Product name
  • Description
    Mouse polyclonal to AFG3L2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

  • Positive control
    • AFG3L2 transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab68023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 89 kDa).


  • Function
    ATP-dependent protease which is essential for axonal development.
  • Tissue specificity
    Ubiquitous. Highly expressed in the cerebellar Purkinje cells.
  • Involvement in disease
    Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) [MIM:610246]. It is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA28 is an autosomal dominant cerebellar ataxia (ADCA) with a slow progressive course and no evidence of sensory involvement or cognitive impairment.
    Defects in AFG3L2 are the cause of spastic ataxia autosomal recessive type 5 (SPAX5) [MIM:614487]. A neurodegenerative disorder characterized by early onset spasticity, peripheral neuropathy, ptosis, oculomotor apraxia, dystonia, cerebellar atrophy, and progressive myoclonic epilepsy.
  • Sequence similarities
    In the N-terminal section; belongs to the AAA ATPase family.
    In the C-terminal section; belongs to the peptidase M41 family.
  • Cellular localization
    Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFG3 (ATPase family gene 3, yeast) like 2 antibody
    • AFG3 ATPase family gene 3 like 2 (yeast) antibody
    • AFG3 ATPase family gene 3 like 2 antibody
    • AFG3 like protein 2 antibody
    • AFG3-like protein 2 antibody
    • AFG32_HUMAN antibody
    • AFG3L2 antibody
    • ATPase family gene 3 like 2 antibody
    • ATPase family gene 3 yeast antibody
    • EC 3.4.24.- antibody
    • FLJ25993 antibody
    • Paraplegin like protein antibody
    • Paraplegin-like protein antibody
    • SCA28 antibody
    • Spinocerebellar ataxia 28 antibody
    see all


  • All lanes : Anti-AFG3L2 antibody (ab68023) at 1/500 dilution

    Lane 1 : AFG3L2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary
    antibody at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 89 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

  • ICC/IF image of ab68023 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68023, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Boutoual R  et al. Defects in the mitochondrial-tRNA modification enzymes MTO1 and GTPBP3 promote different metabolic reprogramming through a HIF-PPAR?-UCP2-AMPK axis. Sci Rep 8:1163 (2018). Read more (PubMed: 29348686) »
  • Meseguer S  et al. microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases. Sci Rep 7:6209 (2017). Read more (PubMed: 28740091) »

See all 4 Publications for this product

Customer reviews and Q&As

I have initiated the replacement order for one vial of ab17995. You should receive it shortly. Please let me know if you need anything further.

Thanks for your email.

Yes, I will be able to send a vial of ab17995 to replace the original vial of ab68023. However I was unable to locate the order in our system. Do you any other order numbers or Abcam order reference numbers associated w...

Read More

Thanks for submitting your completed questionnaire.

I am sorry this vial of ab68023 is giving you trouble. The protocol seems fine and the loading control staining worked well. So it seems there is an issue with the vial of antibody you recei...

Read More

Thank you for your response and yoru comment.

This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab68023. For your information, the internal referen...

Read More

Thank you for your response.

The datasheet suggests using AFG3L2 transfected 293T cell lysate as positive. Furthermore, the Western blot image describes that AFG3L2 transfected 293T cell lysate gave a signal at the expected size (around 80 kD...

Read More

Thank you for your asking and letting us know the enquiry regarding ab68023 .

The antibody has been tested in AFG3L2 transfected 293T cell lysate as the datasheet indicates. We have no data/experience yet in using the antibody on other sample...

Read More

Thank you for your enquiry regarding ab68023 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

Could you please confirm if the customer is using...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (HEK-293, Hela)
Loading amount
20 µg
HEK-293, Hela
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Benny Garfinkel

Verified customer

Submitted Jul 08 2010


Sign up