Overview

  • Product name
    Anti-Aflatoxin antibody [1C6]
  • Description
    Rat monoclonal [1C6] to Aflatoxin
  • Host species
    Rat
  • Specificity
    This antibody reacts with Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1). It does not react with BSA and other irrelevant antigens by ELISA. A competitive binding assay using AFM1 as the binding competitor to compete with AFM1-BSA-125I showed that AFM1 can effectively inhibit the binding of this antibody to AFM1-BSA-125I and the inhibition degree corresponds to the amounts of AFM1 used. The equilibrium constant K of antibody affinity of this product is 109-1011 M-1.
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Aflatoxin M1-BSA conjugate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    1C6
  • Myeloma
    unknown
  • Isotype
    IgG2b
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. This antibody is reactive to purified AFB1 and AF in samples obtained from cereals contaminated with fungus Aspergellus flarus. Therefore, this antibody can be used for construction of an AF detection kit.

Target

  • Relevance
    Aflatoxins are naturally occurring mycotoxins that are produced by many species of Aspergillus.

References

This product has been referenced in:
  • Kanungo L & Bhand S Fluorimetric immunoassay for multianalysis of aflatoxins. J Anal Methods Chem 2013:584964 (2013). ELISA . Read more (PubMed: 24000318) »
  • Parker CO & Tothill IE Development of an electrochemical immunosensor for aflatoxin M1 in milk with focus on matrix interference. Biosens Bioelectron 24:2452-7 (2009). Read more (PubMed: 19167207) »

See all 3 Publications for this product

Publishing research using ab685? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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9 reviews or Q&A

10
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What is the concentration of the product?

Thank you for contacting us. I apologize for the delay in getting back to you.

The concentration of this antibody is 0.94 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

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Please send detection sensitivity of Aflatoxin B1, B2, G1 and G2 and method(s) used (e.g. 50% inhibition in competitive ELISA and incubation duration, etc.) for your Aflatoxin monoclonal antibodies or any others that are not listed on your website.

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Thank you very much for contacting us with your enquiry, and for your patience while I have been in touch with the production labs for more information about our aflatoxin antibodies.

We have three monoclonal antibodies against aflatoxin: ab6...

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Is it possible buy a clone for do ourself monoclonal antibodies? In particular we are interested to Anti-aflatoxin antibody(1C6) - ab685.
Thank you in advance.

Best Regards,

Thank you for contacting us.

We source this product from our collaborator supplier; unfortunately this clone is not produced by Abcam so we can't offer this. We also can not reveal the identity of collaborator supplier which is a proprietary ...

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Can you give us some informations about the eluition buffer that you use in the immunoaffinity purification process? Probably there is a small contamination of some interpherents in your PBS.

Thank you for contacting us.

I have the following information about ab685 Anti-Aflatoxin antibody [1C6]. The antibody was purified with Gamma-Binding Plus Sepharose from GE healthcare. The product was eluted using 0.5M acetic acid pH 3.0. Th...

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en dehors de la question du lot précédent, vous m’aviez promis de revenir avec quelques informations – suggestions quant au stockage de l’anticorps. Avez-vous des données de stabilité spécifiques pour ce produit?



L'anticorps doit être aliquoté sans être diluéet stocké à -20ºC ou -80ºC. Les cycles congélation / décongélation doivent être évités.

Pour une stabilité optimale, nous recommandons un volume minimal des aliquotsde10µl et l'utilisati...

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It is just a simple question. I am a user of this antibody and I was wondering if you can tell me its molecular weight to aid my calculations. Alternatively with your expertise what would you expect its molecular weight to be.

We don't know the exact weight, but it should be similar to the IgG weight or five (5) times that of IgM. In general, it should be between 160-170 kDa. If you have any additional questions, please contact us again.

Can you provide more information of Aflatoxin M1 antibody such as cross reactivity on Aflatoxin B1, B2, G1 and G2? I have an interest in using good Aflatoxin M1 antibody for ELISA test kit.

Unfortunately we do not have any information about this antibody's cross-reactivity with Aflatoxin B2, G1 and G2. I have contacted the originator of the product and they have confirmed that they have only tested this antibody as we specify on the datas...

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Can you provide us with 0.1 up to 0.5 mg labeled (Cy5.5 or Alexa660) "Aflatoxin antibody [1C6] (ab685)"? Thanks a lot in advance!

Thank you for your enquiry. Ab685 is only available as described on the online datasheet and we are unable to specially conjugate it.

Customer is interested in using ab685 for construction of an AF detection kit. Do you have any details about such a kit?

Since there are different iso-forms of the AF protein and your requirement would depend largely on which form you intend on using, we do not have any details for the production of an AF detection kit.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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