Anti-Aflatoxin antibody [1C6] (ab685)

Submit a review Q&A (9)References (4)

Key features and details

  • Rat monoclonal [1C6] to Aflatoxin
  • Suitable for: ELISA
  • Reacts with: Species independent
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Aflatoxin antibody [1C6]

  • Description

    Rat monoclonal [1C6] to Aflatoxin

  • Host species

    Rat

  • Specificity

    This antibody reacts with Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1). It does not react with BSA and other irrelevant antigens by ELISA. A competitive binding assay using AFM1 as the binding competitor to compete with AFM1-BSA-125I showed that AFM1 can effectively inhibit the binding of this antibody to AFM1-BSA-125I and the inhibition degree corresponds to the amounts of AFM1 used. The equilibrium constant K of antibody affinity of this product is 109-1011 M-1.

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Aflatoxin M1-BSA conjugate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Constituent: 0.0268% PBS
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    1C6

  • Myeloma

    unknown

  • Isotype

    IgG2b

  • Light chain type

    unknown

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration. This antibody is reactive to purified AFB1 and AF in samples obtained from cereals contaminated with fungus Aspergellus flarus. Therefore, this antibody can be used for construction of an AF detection kit.
Notes
ELISA
Use at an assay dependent concentration. This antibody is reactive to purified AFB1 and AF in samples obtained from cereals contaminated with fungus Aspergellus flarus. Therefore, this antibody can be used for construction of an AF detection kit.

Target

  • Relevance

    Aflatoxins are naturally occurring mycotoxins that are produced by many species of Aspergillus.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab685? Please let us know so that we can cite the reference in this datasheet.

ab685 has been referenced in 4 publications.

  • Chalyan T  et al. AFM1 Detection in Milk by Fab' Functionalized Si3N4 Asymmetric Mach-Zehnder Interferometric Biosensors. Toxins (Basel) 11:N/A (2019). PubMed: 31337103
  • Kanungo L & Bhand S Fluorimetric immunoassay for multianalysis of aflatoxins. J Anal Methods Chem 2013:584964 (2013). ELISA . PubMed: 24000318
  • Parker CO & Tothill IE Development of an electrochemical immunosensor for aflatoxin M1 in milk with focus on matrix interference. Biosens Bioelectron 24:2452-7 (2009). PubMed: 19167207
  • Parker CO  et al. Electrochemical immunochip sensor for aflatoxin M1 detection. Anal Chem 81:5291-8 (2009). PubMed: 19489595

Customer reviews and Q&As

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1-9 of 9 Abreviews or Q&A

Question

What is the concentration of the product?

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Answer

Thank you for contacting us. I apologize for the delay in getting back to you.

The concentration of this antibody is 0.94 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Please send detection sensitivity of Aflatoxin B1, B2, G1 and G2 and method(s) used (e.g. 50% inhibition in competitive ELISA and incubation duration, etc.) for your Aflatoxin monoclonal antibodies or any others that are not listed on your website.
In addition to the initial inquiry, I’d be interested in knowing the carrier protein used as immunogen and in detection sensitivity evaluation.

Thanks.

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Answer

Thank you very much for contacting us with your enquiry, and for your patience while I have been in touch with the production labs for more information about our aflatoxin antibodies.

We have three monoclonal antibodies against aflatoxin: ab685, ab1017, and ab1985. Here is the information about each of these antibodies:

Ab685: reacts with AFM1 and AFB1. It was raised against AFM1-BSA conjugate, but unfortunately the lab does not have further data regarding the detection sensitivity of the antibody.

Ab1017: reacts with AFB1, and cross-reactivity with other aflatoxins is not known at this time. It was raised against purified AFB1. It has been tested in ELISA at 5 ug/mL against AFB1 coated on wells at 1 ug/mL but the detection sensitivity not been determined.

Ab1985: reacts with AFB1 and AFB2, and it does not cross-react with G1, G2, and M1. It was raised against aflatoxin from Aspergillus flavus conjugated to BSA. The lab has been able to reach the detection limit of 0.2 ng/mL againt AFB1-BSA in competitive ELISA. In this assay, the antibody incubation was performed for 1 hour at 37C and different concentrations of antibody were used.

I hope that this information will be useful, but if you have any further questions or need anything else, please let me know and I'll be happy to help.

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Question

Is it possible buy a clone for do ourself monoclonal antibodies? In particular we are interested to Anti-aflatoxin antibody(1C6) - ab685.
Thank you in advance.

Best Regards,

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Answer

Thank you for contacting us.

We source this product from our collaborator supplier; unfortunately this clone is not produced by Abcam so we can't offer this. We also can not reveal the identity of collaborator supplier which is a proprietary information.

I am sorry I am unable to help in this occasion. Please do not hesitate to contact us if you need any more information.

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Question

Can you give us some informations about the eluition buffer that you use in the immunoaffinity purification process? Probably there is a small contamination of some interpherents in your PBS.

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Answer

Thank you for contacting us.

I have the following information about ab685 Anti-Aflatoxin antibody [1C6]. The antibody was purified with Gamma-Binding Plus Sepharose from GE healthcare. The product was eluted using 0.5M acetic acid pH 3.0. The pH was adjusted with 1M Tris-HCl pH-9.0.

I hope this information is helpful. Please do not hesitate to contact me should you have further questions.

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Question

en dehors de la question du lot précédent, vous m’aviez promis de revenir avec quelques informations – suggestions quant au stockage de l’anticorps. Avez-vous des données de stabilité spécifiques pour ce produit?

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Answer



L'anticorps doit être aliquoté sans être diluéet stocké à -20ºC ou -80ºC. Les cycles congélation / décongélation doivent être évités.

Pour une stabilité optimale, nous recommandons un volume minimal des aliquotsde10µl et l'utilisation de tubes adaptés aux volumes choisis. Par exemple 10µl dans des tubes PCR, pas dans des tubes Eppendorf de 1.5ml.

Stockéà -20ºC l'anticorps sera stable pendant 5 ans, ou 10 anssi stockéà -80ºC.

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Question

It is just a simple question. I am a user of this antibody and I was wondering if you can tell me its molecular weight to aid my calculations. Alternatively with your expertise what would you expect its molecular weight to be.

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Answer

We don't know the exact weight, but it should be similar to the IgG weight or five (5) times that of IgM. In general, it should be between 160-170 kDa. If you have any additional questions, please contact us again.

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Question

Can you provide more information of Aflatoxin M1 antibody such as cross reactivity on Aflatoxin B1, B2, G1 and G2? I have an interest in using good Aflatoxin M1 antibody for ELISA test kit.

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Answer

Unfortunately we do not have any information about this antibody's cross-reactivity with Aflatoxin B2, G1 and G2. I have contacted the originator of the product and they have confirmed that they have only tested this antibody as we specify on the datasheet. I am sorry that we do not have more information.

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Question

Can you provide us with 0.1 up to 0.5 mg labeled (Cy5.5 or Alexa660) "Aflatoxin antibody [1C6] (ab685)"? Thanks a lot in advance!

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Answer

Thank you for your enquiry. Ab685 is only available as described on the online datasheet and we are unable to specially conjugate it.

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Question

Customer is interested in using ab685 for construction of an AF detection kit. Do you have any details about such a kit?

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Answer

Since there are different iso-forms of the AF protein and your requirement would depend largely on which form you intend on using, we do not have any details for the production of an AF detection kit.

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