Overview

  • Product name

  • Description

    Rabbit polyclonal to AFM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 383-617 of Human AFM.

  • Positive control

    • 293T, A431, HeLa, HepG2, NIH 3T3, JC and BCL-1 whole cell lysates; Cal27 xenograft tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab152141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AFAM_HUMAN antibody
    • Afamin antibody
    • Afm antibody
    • ALB 2 antibody
    • ALB2 antibody
    • ALBA antibody
    • ALF antibody
    • Alpha albumin antibody
    • Alpha-Alb antibody
    • Alpha-albumin antibody
    see all

Images

  • All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS PAGE
  • All lanes : Anti-AFM antibody (ab152141) at 1/5000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling AFM with ab152141 at 1/500 dilution. Lower image co-stained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling AFM with ab152141 at 1/500 dilution.

References

ab152141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab152141.
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