Recombinant
RabMAb

Recombinant Anti-AFM antibody [EPR19383] (ab201034)

Overview

  • Product name

    Anti-AFM antibody [EPR19383]
    See all AFM primary antibodies
  • Description

    Rabbit monoclonal [EPR19383] to AFM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human AFM aa 1-250. The exact sequence is proprietary.
    Database link: P43652

  • Positive control

    • WB: Untreated and PNGase F treated mouse serum; Human serum, Human blood, mouse plasma, mouse serum, rat serum, rat plasma and Human plasma.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 69 kDa).

Target

Images

  • All lanes : Anti-AFM antibody [EPR19383] (ab201034) at 1/1000 dilution

    Lane 1 : Untreated mouse serum
    Lane 2 : Mouse serum PNGase F treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 65,87 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    AFM is a glycoprotein, its apparent molecular weight on SDS-PAGE is 87 kDa due to glycosylation. AFM  has 4 potential  sites  for N-Glycosylation and N-glycanase  treatment reduced  the apparent MW to 65 kDa (PMID:7517938).

  • All lanes : Anti-AFM antibody [EPR19383] (ab201034) at 1/1000 dilution

    Lane 1 : Human serum at 20 µg
    Lane 2 : Human blood at 20 µg
    Lane 3 : Mouse plasma at 20 µg
    Lane 4 : Mouse serum at 20 µg
    Lane 5 : Rat serum at 20 µg
    Lane 6 : Rat plasma at 20 µg
    Lane 7 : Human plasma at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 87 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3,4,5 and 7: 30 seconds; Lane 6: 3 seconds.

References

ab201034 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201034.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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