Product nameAFM positive control ChIP primer pair
See all AFM primary antibodies
DescriptionAFM positive control ChIP primer pair
Tested applicationsSuitable for: ChIPmore details
Positive control ChIP-qPCR 5' and 3' primers for AFM gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K9 histone mark. They may also be useful for other histone marks.
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab269259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
FunctionVitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier.
Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.
- Information by UniProt
- AFAM_HUMAN antibody
- Afamin antibody
- Afm antibody
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269259 has not yet been referenced specifically in any publications.