AFM positive control ChIP primer pair (ab269259)
Overview
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Product name
AFM positive control ChIP primer pair -
General notes
Positive control ChIP-qPCR 5' and 3' primers for AFM gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K9 histone mark. They may also be useful for other histone marks.
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
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Tested applications
Suitable for: ChIPmore details
Properties
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Form
Lyophilized -
Storage instructions
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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Research areas
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Function
Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient or across the blood-brain barrier. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AFAM_HUMAN
- Afamin
- Afm
see all
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ChIP |
Use at an assay dependent concentration.
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Notes |
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ChIP
Use at an assay dependent concentration. |
Images
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Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab1220 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first Kb of the transcribed region.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab269259 has not yet been referenced specifically in any publications.