Anti-Aftiphilin antibody (ab122239)
Key features and details
- Rabbit polyclonal to Aftiphilin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aftiphilin antibody -
Description
Rabbit polyclonal to Aftiphilin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: RENNKINRVN ELNSVKEVAL GRSLDNKGDT DGEDQVCVSE ISIVTNRGFS VEKQGLPTLQ QDEFLQSGVQ SK, corresponding to amino acids 262-333 of Human Aftiphilin.
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Positive control
- Human smooth muscle tissue; Human cell line U-2 OS
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General notes
ab122239 is mono-specific.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
ab122239 is mono-specific. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in membrane trafficking. -
Domain
The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself. -
Cellular localization
Cytoplasm. Colocalizes with AP1G1 and clathrin. - Information by UniProt
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Database links
- Entrez Gene: 54812 Human
- SwissProt: Q6ULP2 Human
- Unigene: 655167 Human
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Alternative names
- AFTIN_HUMAN antibody
- Aftiphilin antibody
- Aftiphilin protein antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122239 has not yet been referenced specifically in any publications.