Anti-Aftiphilin antibody - C-terminal (ab175484)

Overview

  • Product name
    Anti-Aftiphilin antibody - C-terminal
    See all Aftiphilin primary antibodies
  • Description
    Rabbit polyclonal to Aftiphilin - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Aftiphilin aa 851-879 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6ULP2

  • Positive control
    • CEM, Jurkat and MCF7 cell line lysates; Human brain tissue; HL60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 102 kDa.

Target

  • Function
    May play a role in membrane trafficking.
  • Domain
    The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
  • Cellular localization
    Cytoplasm. Colocalizes with AP1G1 and clathrin.
  • Information by UniProt
  • Database links
  • Alternative names
    • AFTIN_HUMAN antibody
    • Aftiphilin antibody
    • Aftiphilin protein antibody
    • Aftph antibody
    • MGC33965 antibody
    • Nbla10388 antibody
    see all

Images

  • All lanes : Anti-Aftiphilin antibody - C-terminal (ab175484) at 1/1000 dilution

    Lane 1 : CEM cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : MCF7 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 102 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling Aftiphilin with ab175484 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HL60 cells labeling Aftiphilin with ab175484 antibody at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175484 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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