Product nameAnti-Aftiphilin antibody - C-terminal
See all Aftiphilin primary antibodies
DescriptionRabbit polyclonal to Aftiphilin - C-terminal
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human Aftiphilin aa 851-879 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q6ULP2
- CEM, Jurkat and MCF7 cell line lysates; Human brain tissue; HL60 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab175484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 102 kDa.|
FunctionMay play a role in membrane trafficking.
DomainThe WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself.
Cellular localizationCytoplasm. Colocalizes with AP1G1 and clathrin.
- Information by UniProt
- AFTIN_HUMAN antibody
- Aftiphilin antibody
- Aftiphilin protein antibody
All lanes : Anti-Aftiphilin antibody - C-terminal (ab175484) at 1/1000 dilution
Lane 1 : CEM cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : MCF7 cell line lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 102 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue labeling Aftiphilin with ab175484 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL60 cells labeling Aftiphilin with ab175484 antibody at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175484 has not yet been referenced specifically in any publications.