Recombinant Anti-AG-3 antibody [EPR19606] (ab201464)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19606] to AG-3
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AG-3 antibody [EPR19606]
See all AG-3 primary antibodies -
Description
Rabbit monoclonal [EPR19606] to AG-3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 and T-47D whole cell lysates; human colon cancer, breast cancer and ovary cancer lysates. ICC/IF: MCF7 cells. Flow Cyt (intra): MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19606 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/600.
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ICC/IF |
1/500.
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WB |
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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Notes |
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Flow Cyt (Intra)
1/600. |
ICC/IF
1/500. |
WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
Target
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Sequence similarities
Belongs to the AGR family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 155465 Human
- Omim: 609482 Human
- SwissProt: Q8TD06 Human
- Unigene: 100686 Human
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Alternative names
- AG-3 antibody
- AG3 antibody
- Agr3 antibody
see all
Images
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Lanes 1 & 5 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution
Lane 2 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution
Lanes 3-4 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 3 : Human colon cancer tissue lysate
Lane 4 : Human breast cancer tissue lysate
Lane 5 : Human ovary cancer tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/5: 15 seconds; Lane 2-4: 3 minutes.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab201464 has been referenced in 1 publication.
- Xu Q et al. Anterior Gradient 3 Promotes Breast Cancer Development and Chemotherapy Response. Cancer Res Treat N/A:N/A (2019). PubMed: 31291711