RecombinantRabMAb

Recombinant Anti-AG-3 antibody [EPR19606] (ab201464)

Submit a review Submit a question References (1)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19606] to AG-3
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AG-3 antibody [EPR19606]
    See all AG-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR19606] to AG-3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human AG-3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8TD06

  • Positive control

    • WB: MCF7 and T-47D whole cell lysates; human colon cancer, breast cancer and ovary cancer lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19606

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt 1/600.
WB 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Sequence similarities

    Belongs to the AGR family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • AG-3 antibody
      • AG3 antibody
      • Agr3 antibody
      • AGR3_HUMAN antibody
      • Anterior gradient 3, xenopus, homolog of antibody
      • Anterior gradient homolog 3 antibody
      • Anterior gradient homolog 3 (Xenopus laevis) antibody
      • anterior gradient protein 3 antibody
      • Anterior gradient protein 3 homolog antibody
      • BCMP11 antibody
      • Breast cancer membrane protein 11 antibody
      • Gm888 antibody
      • hAG 3 antibody
      • hAG-3 antibody
      • HAG3 antibody
      • OTTHUMP00000158512 antibody
      • PDIA18 antibody
      • Protein disulfide isomerase family A member 18 antibody
      see all

    Images

    • Western blot - Anti-AG-3 antibody [EPR19606] (ab201464)
      Western blot - Anti-AG-3 antibody [EPR19606] (ab201464)
      Lanes 1 & 5 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution
      Lane 2 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution
      Lanes 3-4 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
      Lane 3 : Human colon cancer lysate
      Lane 4 : Human breast cancer lysate
      Lane 5 : Human ovary cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1/5: 15 seconds; Lane 2-4: 3 minutes.

    • Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] (ab201464)
      Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] (ab201464)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
      -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Flow Cytometry - Anti-AG-3 antibody [EPR19606] (ab201464)
      Flow Cytometry - Anti-AG-3 antibody [EPR19606] (ab201464)

      Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    • Anti-AG-3 antibody [EPR19606] (ab201464)
      Anti-AG-3 antibody [EPR19606] (ab201464)

    References (1)

    Publishing research using ab201464? Please let us know so that we can cite the reference in this datasheet.

    ab201464 has been referenced in 1 publication.

    • Xu Q  et al. Anterior Gradient 3 Promotes Breast Cancer Development and Chemotherapy Response. Cancer Res Treat N/A:N/A (2019). PubMed: 31291711

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab201464.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com