Recombinant Anti-AG-3 antibody [EPR19606] (ab201464)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-AG-3 antibody [EPR19606]
See all AG-3 primary antibodies -
Description
Rabbit monoclonal [EPR19606] to AG-3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human AG-3 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q8TD06 -
Positive control
- WB: MCF7 and T-47D whole cell lysates; human colon cancer, breast cancer and ovary cancer lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19606 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. | |
| Flow Cyt | 1/600. | |
| WB | 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
Target
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Sequence similarities
Belongs to the AGR family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 155465 Human
- Omim: 609482 Human
- SwissProt: Q8TD06 Human
- Unigene: 100686 Human
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Alternative names
- AG-3 antibody
- AG3 antibody
- Agr3 antibody
see all
Images
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![Western blot - Anti-AG-3 antibody [EPR19606] (ab201464)](https://www.abcam.com/ps/products/201/ab201464/Images/ab201464-262126-anti-ag-3-antibody-epr19606-western-blot.jpg)
Western blot - Anti-AG-3 antibody [EPR19606] (ab201464)Lanes 1 & 5 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution
Lane 2 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution
Lanes 3-4 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 3 : Human colon cancer lysate
Lane 4 : Human breast cancer lysate
Lane 5 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/5: 15 seconds; Lane 2-4: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] (ab201464)](https://www.abcam.com/ps/products/201/ab201464/Images/ab201464-262125-anti-ag-3-antibody-epr19606-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] (ab201464)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
![Flow Cytometry - Anti-AG-3 antibody [EPR19606] (ab201464)](https://www.abcam.com/ps/products/201/ab201464/Images/ab201464-262124-anti-ag-3-antibody-epr19606-flow-cytometry.jpg)
Flow Cytometry - Anti-AG-3 antibody [EPR19606] (ab201464)Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xu Q et al. Anterior Gradient 3 Promotes Breast Cancer Development and Chemotherapy Response. Cancer Res Treat N/A:N/A (2019). Read more (PubMed: 31291711) »
