Product nameAnti-AG-3 antibody [EPR19606]
See all AG-3 primary antibodies
DescriptionRabbit monoclonal [EPR19606] to AG-3
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein within Human AG-3 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q8TD06
- WB: MCF7 and T-47D whole cell lysates; human colon cancer, breast cancer and ovary cancer lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab201464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).|
Sequence similaritiesBelongs to the AGR family.
- Information by UniProt
- AG-3 antibody
- AG3 antibody
- Agr3 antibody
Lanes 1 & 5 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution
Lane 2 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution
Lanes 3-4 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lane 3 : Human colon cancer lysate
Lane 4 : Human breast cancer lysate
Lane 5 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/5: 15 seconds; Lane 2-4: 3 minutes.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).
The negative controls are as follows:
-ve control 1: ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.