Recombinant
RabMAb

Recombinant Anti-AG-3 antibody [EPR19606] (ab201464)

Overview

  • Product name

    Anti-AG-3 antibody [EPR19606]
    See all AG-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR19606] to AG-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human AG-3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8TD06

  • Positive control

    • WB: MCF7 and T-47D whole cell lysates; human colon cancer, breast cancer and ovary cancer lysates. ICC/IF: MCF7 cells. Flow Cyt: MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19606
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
Flow Cyt 1/600.
WB 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Sequence similarities

    Belongs to the AGR family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AG-3 antibody
    • AG3 antibody
    • Agr3 antibody
    • AGR3_HUMAN antibody
    • Anterior gradient 3, xenopus, homolog of antibody
    • Anterior gradient homolog 3 antibody
    • Anterior gradient homolog 3 (Xenopus laevis) antibody
    • anterior gradient protein 3 antibody
    • Anterior gradient protein 3 homolog antibody
    • BCMP11 antibody
    • Breast cancer membrane protein 11 antibody
    • Gm888 antibody
    • hAG 3 antibody
    • hAG-3 antibody
    • HAG3 antibody
    • OTTHUMP00000158512 antibody
    • PDIA18 antibody
    • Protein disulfide isomerase family A member 18 antibody
    see all

Images

  • Lanes 1 & 5 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution
    Lane 2 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution
    Lanes 3-4 : Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
    Lane 3 : Human colon cancer lysate
    Lane 4 : Human breast cancer lysate
    Lane 5 : Human ovary cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/5: 15 seconds; Lane 2-4: 3 minutes.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

References

This product has been referenced in:

See 1 Publication for this product

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