Overview

  • Product name

  • Description

    Rabbit polyclonal to AGAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 24-261 of Human AGAP1 (Uniprot: Q9UPQ3-3).

  • Positive control

    • MCF7 whole cell lysate; MDAMB231 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab151480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • Domain

    The PH domain mediates AP-3 binding.
  • Post-translational
    modifications

    Phosphorylated on tyrosines.
  • Cellular localization

    Cytoplasm. Associates with the endocytic compartment.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGAP 1 antibody
    • AGAP-1 antibody
    • Agap1 antibody
    • AGAP1_HUMAN antibody
    • Arf GAP with GTP binding proteinlike ANK repeat and PH domains 1 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with GTPase domain ankyrin repeat and PH domain 1 antibody
    • Centaurin gamma 2 antibody
    • Centaurin-gamma-2 antibody
    • CENTG2 antibody
    • Cnt-g2 antibody
    • GGAP 1 antibody
    • GGAP1 antibody
    • GTP binding and GTPase activating protein 1 antibody
    • GTP-binding and GTPase-activating protein 1 antibody
    • KIAA1099 antibody
    • MGC71657 antibody
    see all

Images

  • Anti-AGAP1 antibody (ab151480) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE
  • Immunohistochemical analysis of paraffin embedded MDAMB231 xenograft tissue, labeling AGAP1 with ab151480 at 1/250 dilution.

References

ab151480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151480.
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