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    agap1-antibody-ab151480.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
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Anti-AGAP1 antibody (ab151480)

  • Datasheet
  • SDS
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Western blot - Anti-AGAP1 antibody (ab151480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGAP1 antibody (ab151480)

Key features and details

  • Rabbit polyclonal to AGAP1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-AGAP1 antibody
    See all AGAP1 primary antibodies
  • Description

    Rabbit polyclonal to AGAP1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 24-261 of Human AGAP1 (Uniprot: Q9UPQ3-3).

  • Positive control

    • MCF7 whole cell lysate; MDAMB231 xenograft tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Xenopus laevis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • Domain

    The PH domain mediates AP-3 binding.
  • Post-translational
    modifications

    Phosphorylated on tyrosines.
  • Cellular localization

    Cytoplasm. Associates with the endocytic compartment.
  • Target information above from: UniProt accession Q9UPQ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 116987 Human
    • Entrez Gene: 347722 Mouse
    • Entrez Gene: 431967 Xenopus laevis
    • Omim: 608651 Human
    • SwissProt: Q9UPQ3 Human
    • SwissProt: Q8BXK8 Mouse
    • SwissProt: Q6NRL1 Xenopus laevis
    • Unigene: 435039 Human
    • Unigene: 291135 Mouse
    • Unigene: 12652 Xenopus laevis
    see all
  • Alternative names

    • AGAP 1 antibody
    • AGAP-1 antibody
    • Agap1 antibody
    • AGAP1_HUMAN antibody
    • Arf GAP with GTP binding proteinlike ANK repeat and PH domains 1 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 antibody
    • ArfGAP with GTPase domain ankyrin repeat and PH domain 1 antibody
    • Centaurin gamma 2 antibody
    • Centaurin-gamma-2 antibody
    • CENTG2 antibody
    • Cnt-g2 antibody
    • GGAP 1 antibody
    • GGAP1 antibody
    • GTP binding and GTPase activating protein 1 antibody
    • GTP-binding and GTPase-activating protein 1 antibody
    • KIAA1099 antibody
    • MGC71657 antibody
    see all

Images

  • Western blot - Anti-AGAP1 antibody (ab151480)
    Western blot - Anti-AGAP1 antibody (ab151480)
    Anti-AGAP1 antibody (ab151480) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGAP1 antibody (ab151480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGAP1 antibody (ab151480)
    Immunohistochemical analysis of paraffin embedded MDAMB231 xenograft tissue, labeling AGAP1 with ab151480 at 1/250 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab151480? Please let us know so that we can cite the reference in this datasheet.

    ab151480 has not yet been referenced specifically in any publications.

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