Anti-AGAP1 antibody (ab151480)
Key features and details
- Rabbit polyclonal to AGAP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AGAP1 antibody
See all AGAP1 primary antibodies -
Description
Rabbit polyclonal to AGAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 24-261 of Human AGAP1 (Uniprot: Q9UPQ3-3).
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Positive control
- MCF7 whole cell lysate; MDAMB231 xenograft tissue
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Xenopus laevis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
GTPase-activating protein for ARF1 and, to a lesser extent, ARF5. Directly and specifically regulates the adapter protein 3 (AP-3)-dependent trafficking of proteins in the endosomal-lysosomal system. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the centaurin gamma-like family.
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain. -
Domain
The PH domain mediates AP-3 binding. -
Post-translational
modificationsPhosphorylated on tyrosines. -
Cellular localization
Cytoplasm. Associates with the endocytic compartment. - Information by UniProt
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Database links
- Entrez Gene: 116987 Human
- Entrez Gene: 347722 Mouse
- Entrez Gene: 431967 Xenopus laevis
- Omim: 608651 Human
- SwissProt: Q9UPQ3 Human
- SwissProt: Q8BXK8 Mouse
- SwissProt: Q6NRL1 Xenopus laevis
- Unigene: 435039 Human
see all -
Alternative names
- AGAP 1 antibody
- AGAP-1 antibody
- Agap1 antibody
see all
Images
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Western blot - Anti-AGAP1 antibody (ab151480)Anti-AGAP1 antibody (ab151480) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGAP1 antibody (ab151480)Immunohistochemical analysis of paraffin embedded MDAMB231 xenograft tissue, labeling AGAP1 with ab151480 at 1/250 dilution.
Protocols
References (0)
ab151480 has not yet been referenced specifically in any publications.
