Agarose Anti-6X His tag® antibody (ab1231)
Key features and details
- Agarose Goat polyclonal to 6X His tag®
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Agarose
- Isotype: IgG
Overview
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Product name
Agarose Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Agarose Goat polyclonal to 6X His tag® -
Host species
Goat -
Conjugation
Agarose -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab1231 has been referenced in 1 publication.
- Ma X et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970