Key features and details
- Agarose Goat polyclonal to 6X His tag®
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Agarose
- Isotype: IgG
Product nameAgarose Anti-6X His tag® antibody
See all 6X His tag® primary antibodies
DescriptionAgarose Goat polyclonal to 6X His tag®
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Species independent
General notesHIS-TAG® is a trademark of EMD Biosciences, Inc.
Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 0.58% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.
Use at an assay dependent concentration. Use 20-40 µl of gel slurry per 0.1-1 mg of protein lysate or extract.
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab1231 has been referenced in 1 publication.
- Ma X et al. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Elife 8:N/A (2019). PubMed: 30652970