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  1. Link

    agarose-ha-tag-antibody-ab214758.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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Agarose Anti-HA tag antibody (ab214758)

  • Datasheet
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Key features and details

  • Agarose Goat polyclonal to HA tag
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

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Overview

  • Product name

    Agarose Anti-HA tag antibody
    See all HA tag primary antibodies
  • Description

    Agarose Goat polyclonal to HA tag
  • Host species

    Goat
  • Conjugation

    Agarose
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. HA tag epitope conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    0.1 mg antibody/0.4 ml of 50% gel slurry containing 0.2 ml agarose.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab214758 was affinity purified using the peptide immobilized on solid support. Goat anti-HA affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag

Associated products

  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.

Use 15 - 25 μl of gel slurry per 0.1 - 1 mg lysate of cell extract.

Notes
IP
Use at an assay dependent concentration.

Use 15 - 25 μl of gel slurry per 0.1 - 1 mg lysate of cell extract.

Target

  • Relevance

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names

    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab214758? Please let us know so that we can cite the reference in this datasheet.

ab214758 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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