Agarose Anti-HA tag antibody (ab214758)
Key features and details
- Agarose Goat polyclonal to HA tag
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Agarose
- Isotype: IgG
Overview
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Product name
Agarose Anti-HA tag antibody
See all HA tag primary antibodies -
Description
Agarose Goat polyclonal to HA tag -
Host species
Goat -
Conjugation
Agarose -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. HA tag epitope conjugated to KLH.
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General notes
0.1 mg antibody/0.4 ml of 50% gel slurry containing 0.2 ml agarose.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab214758 was affinity purified using the peptide immobilized on solid support. Goat anti-HA affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
Use 15 - 25 μl of gel slurry per 0.1 - 1 mg lysate of cell extract. |
Notes |
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IP
Use at an assay dependent concentration. Use 15 - 25 μl of gel slurry per 0.1 - 1 mg lysate of cell extract. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab214758 has been referenced in 1 publication.
- Carotti S et al. Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver. Cell Death Dis 12:1092 (2021). PubMed: 34795230