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    agarose-myc-tag-antibody-ab1253.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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Agarose Anti-Myc tag antibody (ab1253)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (9)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Agarose Goat polyclonal to Myc tag
  • Suitable for: IP
  • Conjugation: Agarose
  • Isotype: IgG

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Overview

  • Product name

    Agarose Anti-Myc tag antibody
    See all Myc tag primary antibodies
  • Description

    Agarose Goat polyclonal to Myc tag
  • Host species

    Goat
  • Conjugation

    Agarose
  • Tested applications

    Suitable for: IPmore details
  • Immunogen

    Full length native protein

    EQKLISEEDL

    (c-myc) (purified): conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Agarose Conjugates

Associated products

  • Immunizing Peptide (Blocking)

    • Human c-Myc peptide (ab13837)
  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

Applications

Our Abpromise guarantee covers the use of ab1253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

Protocols

  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (9)

    Publishing research using ab1253? Please let us know so that we can cite the reference in this datasheet.

    ab1253 has been referenced in 9 publications.

    • Katoh I  et al. C-terminal a Domain of p63 Binds to p300 to Coactivate ß-Catenin. Neoplasia 21:494-503 (2019). PubMed: 30986748
    • Cai L  et al. Identification of a genetic interaction between the tumor suppressor EAF2 and the retinoblastoma protein (Rb) signaling pathway in C. elegans and prostate cancer cells. Biochem Biophys Res Commun 447:292-8 (2014). PubMed: 24727455
    • Carballo JA  et al. Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery. PLoS Genet 9:e1003545 (2013). IP . PubMed: 23825959
    • Warrington SJ  et al. The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2. Development 140:1045-54 (2013). IP . PubMed: 23364328
    • Aavikko M  et al. Loss of SUFU function in familial multiple meningioma. Am J Hum Genet 91:520-6 (2012). PubMed: 22958902
    • Isoda M  et al. Dynamic regulation of Emi2 by Emi2-bound Cdk1/Plk1/CK1 and PP2A-B56 in meiotic arrest of Xenopus eggs. Dev Cell 21:506-19 (2011). PubMed: 21871841
    • Ohe M  et al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). IP . PubMed: 20089832
    • Epting D  et al. The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish. Circ Res 107:45-55 (2010). IP . PubMed: 20466982
    • Thoma C  et al. Enhancement of IRES-mediated translation of the c-myc and BiP mRNAs by the poly(A) tail is independent of intact eIF4G and PABP. Mol Cell 15:925-35 (2004). PubMed: 15383282

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Myc tag antibody (Agarose)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Trypanosoma brucei Cell lysate - other (Abcam beads (ab1253) Anti-myc developed in goat) 1)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    2.5 µg
    Specification
    Abcam beads (ab1253) Anti-myc developed in goat) 1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 17 2020

    Immunoprecipitation abreview for Anti-Myc tag antibody (Agarose)

    Excellent
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK 293T)
    Total protein in input
    25 µg
    Specification
    HEK 293T
    Immuno-precipitation step
    Other - Pre-bound agarose
    Read More

    Dr. Jonathan Rud

    Verified customer

    Submitted Dec 28 2009

    Immunoprecipitation abreview for Anti-Myc tag antibody (Agarose)

    Average
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa)
    Specification
    HeLa
    Immuno-precipitation step
    Other
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 03 2005

    Question

    Is there an IP protocol that should be used with this antibody? Specifically, customer would like to know if the beads should be washed prior to use and if so, with what buffer?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2005

    Answer

    Thank you for your enquiry. You don't need to wash the beads prior to using, and perform the IP at 4C. Also, use 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Below is a general IP protocol that the originator of this antibody sent that you can use as a guideline. If you have any additional questions, please contact us again. Immunoprecipitation Protocol For use with Antibodies Immobilized on Agarose: A. To microcentrifuge tube add: 1) 15 to 25 ul of slurry of agarose immobilized Ab (3.75 to 6.125 ug Ab) 2)500 ul 2x Stringent IP buffer*: 100 mM Tris-HCl (pH 8.0) 1000 mM Nacl Chelating agents: 2 mM EDTA and/or 2 mM EDTA Detergents: 2 % IGEPAL CA-630 (replacement for NP40) 0.2 % SDS 2 % Deoxycholate Protease Inhibitors: 20 ug/ml Aprotinin 20 ug/ml Leupeptin 2 mM PMSF or 10 ug/ml Pepstatin A or 8 mM AEBSF Phosphatase Inhibitors – If Necessary: 200 mM NaF 2 mM Na3VO4 50 mM ?-Glycerophosphate 3)Cell lysate (0.1 to 1.0 mg total protein) 4)diH2O to a total volume of 1 ml B. Vortex and incubate for between 1 and 24 h at 4 deg. Turning tubes on a rotating platform wheel allows continuous mixing. C. Centrifuge (16,000 x g, 3 to 5 min), aspirate and discard supernatant D. Wash pellet in 1x IP buffer 3 to 6 times. Washing consists of resuspending pellet, centrifuging (16,000 x g, 3 to 5 min), aspirating and discarding supernatant. E. After last wash, resuspend pellet in 30 ul 2x Electrophoresis Sample Buffer, boil 5 min. F. Centrifuge, load supernatant onto SDS-PAGE gel, electroblot onto PVDF membrane and perform Western Blot Analysis. *Buffers for Immunoprecipitation Assays For analysis of a single protein without associated proteins, the use of high ionic strength, strong detergents, and rigorous washing of the immunoprecipitate are recommended. One suitable buffer is the Strigent IP buffer outlined above. For co-precipitation of associated proteins, the use of near physiological ionic strength and gentle detergents is recommended. One suitable buffer is the Lenient IP buffer outlined below. The buffers outlined represent two extremes. The optimal buffer for ones given application must be empirically defined. This buffer may be of intermediate ionic strength and/or detergent composition. 1x Lenient IP buffer: 50 mM Tris-HCl (pH 8.0) 100 mM Nacl Chelating agents: 1 mM EDTA and/or 1 mM EGTA Detergents: 0.5 % IGEPAL CA-630 (replacement for Nonidet P40) Protease Inhibitors: 10 ug/ml Aprotinin 10 ug/ml Leupeptin 1 mM PMSF or 5 ug/ml Pepstatin A or 4 mM AEBSF Phosphatase Inhibitors – If Necessary: 100 mM NaF 1 mM Na3VO4 50 mM ?-Glycerophosphate

    Read More

    Abcam Scientific Support

    Answered on Aug 08 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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