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  1. Link

    agarose-myc-tag-antibody-ab1253.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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Agarose Anti-Myc tag antibody (ab1253)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (12)

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Key features and details

  • Agarose Goat polyclonal to Myc tag
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

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Overview

  • Product name

    Agarose Anti-Myc tag antibody
    See all Myc tag primary antibodies
  • Description

    Agarose Goat polyclonal to Myc tag
  • Host species

    Goat
  • Conjugation

    Agarose
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length native protein (purified) corresponding to Human Myc tag conjugated to keyhole limpet haemocyanin.
    Database link: P01106
    (Peptide available as ab13837)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Agarose Conjugates

Associated products

  • Immunizing Peptide (Blocking)

    • Human c-Myc peptide (ab13837)
  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (2)
Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.
Notes
IP
Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

Protocols

  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (12)

Publishing research using ab1253? Please let us know so that we can cite the reference in this datasheet.

ab1253 has been referenced in 12 publications.

  • Strutt H & Strutt D DAnkrd49 and Bdbt act via Casein kinase Ie to regulate planar polarity in Drosophila. PLoS Genet 16:e1008820 (2020). PubMed: 32750048
  • Huang Z  et al. PTPN2 regulates the activation of KRAS and plays a critical role in proliferation and survival of KRAS-driven cancer cells. J Biol Chem 295:18343-18354 (2020). PubMed: 33122197
  • Bucher ML  et al. Acquired dysregulation of dopamine homeostasis reproduces features of Parkinson's disease. NPJ Parkinsons Dis 6:34 (2020). PubMed: 33298952
  • Katoh I  et al. C-terminal a Domain of p63 Binds to p300 to Coactivate ß-Catenin. Neoplasia 21:494-503 (2019). PubMed: 30986748
  • Cai L  et al. Identification of a genetic interaction between the tumor suppressor EAF2 and the retinoblastoma protein (Rb) signaling pathway in C. elegans and prostate cancer cells. Biochem Biophys Res Commun 447:292-8 (2014). PubMed: 24727455
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Myc tag antibody (Agarose)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Trypanosoma brucei Cell lysate - other (Abcam beads (ab1253) Anti-myc developed in goat) 1)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
2.5 µg
Specification
Abcam beads (ab1253) Anti-myc developed in goat) 1
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 17 2020

Immunoprecipitation abreview for Anti-Myc tag antibody (Agarose)

Excellent
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T)
Total protein in input
25 µg
Specification
HEK 293T
Immuno-precipitation step
Other - Pre-bound agarose
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Jonathan Rud

Verified customer

Submitted Dec 28 2009

Immunoprecipitation abreview for Anti-Myc tag antibody (Agarose)

Average
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Immuno-precipitation step
Other
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 03 2005

Question

Is there an IP protocol that should be used with this antibody? Specifically, customer would like to know if the beads should be washed prior to use and if so, with what buffer?

Read More

Abcam community

Verified customer

Asked on Aug 08 2005

Answer

Thank you for your enquiry. You don't need to wash the beads prior to using, and perform the IP at 4C. Also, use 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Below is a general IP protocol that the originator of this antibody sent that you can use as a guideline. If you have any additional questions, please contact us again. Immunoprecipitation Protocol For use with Antibodies Immobilized on Agarose: A. To microcentrifuge tube add: 1) 15 to 25 ul of slurry of agarose immobilized Ab (3.75 to 6.125 ug Ab) 2)500 ul 2x Stringent IP buffer*: 100 mM Tris-HCl (pH 8.0) 1000 mM Nacl Chelating agents: 2 mM EDTA and/or 2 mM EDTA Detergents: 2 % IGEPAL CA-630 (replacement for NP40) 0.2 % SDS 2 % Deoxycholate Protease Inhibitors: 20 ug/ml Aprotinin 20 ug/ml Leupeptin 2 mM PMSF or 10 ug/ml Pepstatin A or 8 mM AEBSF Phosphatase Inhibitors – If Necessary: 200 mM NaF 2 mM Na3VO4 50 mM ?-Glycerophosphate 3)Cell lysate (0.1 to 1.0 mg total protein) 4)diH2O to a total volume of 1 ml B. Vortex and incubate for between 1 and 24 h at 4 deg. Turning tubes on a rotating platform wheel allows continuous mixing. C. Centrifuge (16,000 x g, 3 to 5 min), aspirate and discard supernatant D. Wash pellet in 1x IP buffer 3 to 6 times. Washing consists of resuspending pellet, centrifuging (16,000 x g, 3 to 5 min), aspirating and discarding supernatant. E. After last wash, resuspend pellet in 30 ul 2x Electrophoresis Sample Buffer, boil 5 min. F. Centrifuge, load supernatant onto SDS-PAGE gel, electroblot onto PVDF membrane and perform Western Blot Analysis. *Buffers for Immunoprecipitation Assays For analysis of a single protein without associated proteins, the use of high ionic strength, strong detergents, and rigorous washing of the immunoprecipitate are recommended. One suitable buffer is the Strigent IP buffer outlined above. For co-precipitation of associated proteins, the use of near physiological ionic strength and gentle detergents is recommended. One suitable buffer is the Lenient IP buffer outlined below. The buffers outlined represent two extremes. The optimal buffer for ones given application must be empirically defined. This buffer may be of intermediate ionic strength and/or detergent composition. 1x Lenient IP buffer: 50 mM Tris-HCl (pH 8.0) 100 mM Nacl Chelating agents: 1 mM EDTA and/or 1 mM EGTA Detergents: 0.5 % IGEPAL CA-630 (replacement for Nonidet P40) Protease Inhibitors: 10 ug/ml Aprotinin 10 ug/ml Leupeptin 1 mM PMSF or 5 ug/ml Pepstatin A or 4 mM AEBSF Phosphatase Inhibitors – If Necessary: 100 mM NaF 1 mM Na3VO4 50 mM ?-Glycerophosphate

Read More

Abcam Scientific Support

Answered on Aug 08 2005

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