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    agarose-t7-tag-antibody-ab1230.pdf

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Tags & Cell Markers Epitope Tags T7 Tag
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Agarose Anti-T7 tag® antibody (ab1230)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Agarose Goat polyclonal to T7 tag®
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

Overview

  • Product name

    Agarose Anti-T7 tag® antibody
    See all T7 tag® primary antibodies
  • Description

    Agarose Goat polyclonal to T7 tag®
  • Host species

    Goat
  • Conjugation

    Agarose
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Goats were immunized with

    MASMTGGQQMG

    (T7) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    T7-TAG® is a trademark of EMD Biosciences, Inc.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • T7 Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Agarose Conjugates

Associated products

  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

Applications

Our Abpromise guarantee covers the use of ab1230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Target

  • Relevance

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

Protocols

  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab1230? Please let us know so that we can cite the reference in this datasheet.

    ab1230 has been referenced in 2 publications.

    • Piccinni E  et al. Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim Pol 58:529-34 (2011). IP . PubMed: 22068108
    • MacPherson MJ  et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). IP . PubMed: 19029252

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Hi,
    I would like to check if this product, Anti-T7 tag antibody (Agarose) (ref no.ab1230), is used for purification of T7-tagged protein. If so, is there any protocol which I can refer to. In addition, what is the cost and availability of this product?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for contacting us.

    Indeed, we can confirm that Agarose-conjugated anti-T7 tag® antibodyab1230 can be used to purify (or pull-down) T7-tagged proteins, as it has been tested and is guaranteed to work in immunoprecipitation (IP).

    For a protocol you can refer to the publication by MacPherson who used thisproduct(see attached, and datasheet). For IP protocols in general our protocols website might be of interest for you: https://www.abcam.com/index.html?pageconfig=popular_protocols

    We currently have this product in stock. For further details on pricing and shipping time please contact our distributor in your country. By choosing your country in the top right corner of our website the contact details will be shown on each datasheet, e.g. for Australia it is currently

    Sapphire Bioscience Pty Ltd
    126 Cope Street
    Waterloo - NSW
    2017
    Australia
    tel +61296982022
    Fax +61296981022
    Website http://www.sapphirebioscience.com
    Email mailto:sales@sapphirebioscience.com
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Question

    DESCRIPTION OF THE PROBLEM High background and non-specific protein binding to agarose SAMPLE total cell lysate PRIMARY ANTIBODY T7 antibody from Novagen DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED T7 antibody ANTIBODY STORAGE CONDITIONS 4? SAMPLE PREPARATION RIPA buffer MATRIX USED .. DETERGENT 1mg protein /60ul agarose AMOUNT OF PROTEIN LOADED Reducing gel(SDS-PAGE) 12% ELECTROPHORESIS/GEL CONDITIONS 80V, 2hr SECONDARY ANTIBODY Amersham/Mouse/1:2000/1hr HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Preclearing with various agarose

    Read More

    Abcam community

    Verified customer

    Asked on Feb 05 2007

    Answer

    Thank you for contacting us for technical support with ab1230 on behalf of your customer. Unfortunately it is very hard for me to understand the customer's protocol and results as limited information was provided. Can you please explain what the different lanes are: -what is the positive control? -what is the difference between the two membrane images? (the lanes IP and Total cell lysate don't look the same at all between the two images, I am very confused) -what does "Rx" mean? I would like also to know more protocol information please: -how was the T7 antibody used in WB used: dilution, buffer, incubation time. Is there a positive control for this antibody? -what protease inhibitors were used in the RIPA buffer? -how long was the lysate mixed with the antibody/agarose slurry? -how were the agarose beads uncoupled from the antibody? -what blocking buffer was used: for the beads and the membrane? The more information you can provide the better it will be for me to assist your customer, thank you in advance,

    Read More

    Abcam Scientific Support

    Answered on Feb 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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