Agarose Anti-T7 tag® antibody (ab1230)
Key features and details
- Agarose Goat polyclonal to T7 tag®
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Agarose
- Isotype: IgG
Overview
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Product name
Agarose Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
Agarose Goat polyclonal to T7 tag® -
Host species
Goat -
Conjugation
Agarose -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Goats were immunized with
MASMTGGQQMG
(T7) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. -
General notes
Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
T7-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 0.58% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
Target
-
Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab1230 has been referenced in 3 publications.
- Jiang T et al. Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot 71:7073-7087 (2020). PubMed: 32853383
- Piccinni E et al. Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim Pol 58:529-34 (2011). IP . PubMed: 22068108
- MacPherson MJ et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). IP . PubMed: 19029252