Agarose Anti-V5 tag antibody (ab1229)
Key features and details
- Agarose Goat polyclonal to V5 tag
- Suitable for: IP
- Reacts with: Species independent
- Conjugation: Agarose
- Isotype: IgG
Overview
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Product name
Agarose Anti-V5 tag antibody
See all V5 tag primary antibodies -
Description
Agarose Goat polyclonal to V5 tag -
Host species
Goat -
Conjugation
Agarose -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 0.58% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP | (1) |
Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.
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Notes |
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IP
Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab1229 has been referenced in 17 publications.
- Zhou YT et al. A Species-Correlated Transitional Residue D132 on Human FMRP Plays a Role in Nuclear Localization via an RNA-Dependent Interaction With PABP1. Neuroscience 404:282-296 (2019). PubMed: 30742966
- Cockman EM et al. Identification of the Selenoprotein S Positive UGA Recoding (SPUR) element and its position-dependent activity. RNA Biol 16:1682-1696 (2019). PubMed: 31432740
- Greenberg RS et al. Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Cell 178:1421-1436.e24 (2019). PubMed: 31491386
- Pickar-Oliver A et al. Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells. Nat Biotechnol 37:1493-1501 (2019). PubMed: 31548729
- Liu T et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). PubMed: 29526322