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    agarose-v5-tag-antibody-ab1229.pdf

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Tags & Cell Markers Epitope Tags V5 Tag
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Agarose Anti-V5 tag antibody (ab1229)

  • Datasheet
  • SDS
Reviews (1)Q&A (8)References (17)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Agarose Goat polyclonal to V5 tag
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

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Overview

  • Product name

    Agarose Anti-V5 tag antibody
    See all V5 tag primary antibodies
  • Description

    Agarose Goat polyclonal to V5 tag
  • Host species

    Goat
  • Conjugation

    Agarose
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length native

    GKPIPNPLLGLDST

    (V5) (purified): conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • V5 Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Agarose Conjugates
    • Signal Transduction
    • Antibodies
    • v5 tag

Associated products

  • Immunizing Peptide (Blocking)

    • V5 tag peptide (ab15829)
  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (1)
Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.
Notes
IP
Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

Target

  • Relevance

    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names

    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Protocols

  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (17)

Publishing research using ab1229? Please let us know so that we can cite the reference in this datasheet.

ab1229 has been referenced in 17 publications.

  • Zhou YT  et al. A Species-Correlated Transitional Residue D132 on Human FMRP Plays a Role in Nuclear Localization via an RNA-Dependent Interaction With PABP1. Neuroscience 404:282-296 (2019). PubMed: 30742966
  • Cockman EM  et al. Identification of the Selenoprotein S Positive UGA Recoding (SPUR) element and its position-dependent activity. RNA Biol 16:1682-1696 (2019). PubMed: 31432740
  • Greenberg RS  et al. Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Cell 178:1421-1436.e24 (2019). PubMed: 31491386
  • Pickar-Oliver A  et al. Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells. Nat Biotechnol 37:1493-1501 (2019). PubMed: 31548729
  • Liu T  et al. Glycosylation controls sodium-calcium exchanger 3 sub-cellular localization during cell cycle. Eur J Cell Biol 97:190-203 (2018). PubMed: 29526322
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

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Immunoprecipitation abreview for Anti-V5 tag antibody (Agarose)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Immuno-precipitation step
Other - ab1229
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Treatment
transfected with plasmid DNA
Total protein in input
5e+006 cells
Read More

Ms. Christina Engel

Verified customer

Submitted Oct 10 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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