Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6.
Expressed in brain.
Belongs to the peptidase M14 family.
Cytoplasm > cytosol. Nucleus. Mainly cytoplasmic. Slight accumulation in the nucleus is observed.
Immunohistochemical analysis in formalin fixed, paraffin embedded Human brain tissue labeling AGBL5 with ab170541 at 1/50, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-AGBL5 antibody - N-terminal (ab170541)
Anti-AGBL5 antibody - N-terminal (ab170541) at 1/100 dilution + K562 cell lysate at 35 µg
Secondary Anti-rabbit HRP conjugated antibody at 1/10000 dilution