• Product name
  • Description
    Rabbit polyclonal to AGE
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Advanced Glycation End Products (BSA-AGE and HSA-AGE)

  • Positive control
    • Human lens, arteriosclerotic plaques
  • General notes

    ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.


Our Abpromise guarantee covers the use of ab23722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Can be blocked with Native Cow AGE-BSA protein (ab129535).
ICC/IF Use a concentration of 5 µg/ml.
ELISA Use a concentration of 1 µg/ml.
IHC-Fr 1/2000.
IHC-P 1/10000. PubMed: 19223295


  • Relevance
    The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
  • Cellular localization
    Cell Membrane and Secreted
  • Alternative names
    • Advanced glycation end products antibody
    • AGEs antibody


  • All lanes : Anti-AGE antibody (ab23722) at 1/1000 dilution

    Lane 1 : Mouse Brain Lysate(Brain and Hippocampus) at 25 µg
    Lane 2 : Glycated BSA at 15 µg

    All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/800 dilution

    Performed under reducing conditions.

    12% SDS-PAGE gel run using denaturing conditions. Membrane blocked with 5% BSA for 30 minutes at room temperature. Primary antibody diluted in 5% BSA in TBS-T and incubated for 20 hours in a fridge

    See Abreview

  • ab23722 staining AGE in Rat aoratic tissue sections showing the effects of diabetes and Ator on AGE expression by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin. Samples were incubated with primary antibody (1/10000) overnight at 4°C. A secondary antibody Fab fragment was used for 1 hour at room temperature.

    The arrows indicated the sites of antibody staining. NC=normal controls, DM=streptozotocin-induced diabetic rats.

  • ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab23722 (1µg/ml) staining AGE in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of both cell membrane and cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Liu YW  et al. Neuroprotection of quercetin on central neurons against chronic high glucose through enhancement of Nrf2/ARE/glyoxalase-1 pathway mediated by phosphorylation regulation. Biomed Pharmacother 109:2145-2154 (2019). Read more (PubMed: 30551472) »
  • Hamasaki S  et al. Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis. Sci Rep 8:5901 (2018). Read more (PubMed: 29651042) »
See all 41 Publications for this product

Customer reviews and Q&As

1-10 of 27 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (Dermal fibroblasts)
Loading amount
1.8 µg
Dermal fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Mark Fowler

Verified customer

Submitted Jul 20 2006

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Antigen Retrival solution EDTA buffer pH9
Blocking step
No blocking step used for 15 minute(s) · Concentration: 0.03 · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jul 10 2017


We have not tested the cross reactivity with ab23722 and the other types of AGEs that you mentioned. Only BSA-AGE and HSA-AGE have been tested, and the antibody shoes minimial cross reactivity (< 1%) with BSA and HSA alone.

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This antibody is specific to AGE which is not species specific. Therefore, this antibody can be used in all species including pig.

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This antibody will detect endogenous and artificial AGEs. Mainly in certain diseases as diabetes, arteriosclerosis and Modbus Alzheimer AGE has been detected in various tissues (eye, kidney, artery, brain etc.). In principle all proteins can be affected, not only albumins.

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Western blot
Mouse Tissue lysate - whole (Brain: Cortex and Hippocampus in a mix)
Loading amount
25 µg
Brain: Cortex and Hippocampus in a mix
Gel Running Conditions
Reduced Denaturing (12 % SDS-PAGE gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Miss. Josephine Böhme

Verified customer

Submitted Apr 09 2013


The immunogen for ab23722 was prepared as follows:
Under sterile conditions a mixture of BSA and HSA was incubated with D-Glucose in phosphate buffer pH 7.4 at 37°C for some weeks. Low-molecular weight products and D-Glucose were eliminated by chromatography and dialyses against PBS pH 7.4. This final product was used for immunization of rabbits.

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Thank you for contacting us.

Regarding ab62623, Stressed tissue such as Alzheimer's affected neurons. or Human brain pre treated with 10ug proteinase K for 40mins at 37oC are suitable positive controls.

Regarding ab23722, human colon tissue was tested successfully.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Ablaufdatum: 12. April 2013
Wert: EURO 410.-

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab23722 in WB zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer ABTRIAL-00000, deckt den Wert von ab23722 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in WB eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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