Recombinant Anti-Aggrecan antibody [6-B-4] (ab3778)
![Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)](https://www.abcam.com/ps/products/3/ab3778/Images/ab3778-469968-AntiAggrecan-antibody-westernblot.jpg "Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [6-B-4] to Aggrecan
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Aggrecan antibody [6-B-4]
See all Aggrecan primary antibodies -
Description
Mouse monoclonal [6-B-4] to Aggrecan -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant human Aggrecan protein. C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675). IHC-P: Human cartilage sections.
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General notes
This product has switched from a hybridoma to recombinant production method on 08th October 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.04% PBS -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody detects aggrecan metabolites (intact or matrix protease-catabolised) in human synovial fluid samples. -
Clonality
Monoclonal -
Clone number
6-B-4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 250 kDa.
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| IHC-P | (3) |
Use a concentration of 0.05 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 250 kDa. |
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IHC-P
Use a concentration of 0.05 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
Cellular localization
Secreted; extracellular matrix. -
Database links
- Entrez Gene: 176 Human
- Omim: 155760 Human
- SwissProt: P16112 Human
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Alternative names
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
Images
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Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)Anti-Aggrecan antibody [6-B-4] (ab3778) at 1/1000 dilution + C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675) 15ng
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 250 kDaBuffer and Concentration: 2% BSA/TBST
Exposure time: 180 seconds
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![Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)](https://www.abcam.com/ps/products/3/ab3778/Images/ab3778-414502-AntiAggrecan-antibody-6B4-western-blot-recombinant-protein-human.jpg)
Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)ab3778 was shown to react with recombinant human Aggrecan protein in western blot.
Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1µg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)](https://www.abcam.com/ps/products/3/ab3778/Images/ab3778-414504-AntiAggrecan-antibody-6B4-immunohistochemistry-cartilage-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)Secreted staining of cartilage cells.
Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)](https://www.abcam.com/ps/products/3/ab3778/Images/ab3778-414503-AntiAggrecan-antibody-6B4-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)No staining in negative control tissue (human normal liver).
Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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![Anti-Aggrecan antibody [6-B-4] (ab3778)](https://www.abcam.com/ps/products/3/ab3778/Images/ab3778-9-benefits-of-recombinant-antibodies.png)
Anti-Aggrecan antibody [6-B-4] (ab3778)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (129)
ab3778 has been referenced in 129 publications.
- Zhao K et al. Acid-sensing ion channels regulate nucleus pulposus cell inflammation and pyroptosis via the NLRP3 inflammasome in intervertebral disc degeneration. Cell Prolif 54:e12941 (2021). PubMed: 33111436
- Lu X et al. miR-335-5P contributes to human osteoarthritis by targeting HBP1. Exp Ther Med 21:109 (2021). PubMed: 33335572
- Guo Q et al. STING promotes senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-?B signaling pathway. Cell Death Dis 12:13 (2021). PubMed: 33414452
- Li C et al. LncRNA NEAT1 promotes nucleus pulposus cell matrix degradation through regulating Nrf2/ARE axis. Eur J Med Res 26:11 (2021). PubMed: 33478594
- Wang W et al. Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-ß/SMAD2 signaling. Arthritis Res Ther 23:84 (2021). PubMed: 33726807