Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (Chondrocytes and cartilage tissue)
Permeabilization
No
Specification
Chondrocytes and cartilage tissue
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2.5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA
Dilution
1/300
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Anti-Mouse IgG (whole molecule) F(ab')2 fragment–F
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Sheep
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
the secondary was tested alone both on sheep tissue as well chondrocytes to see no crossreaction
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Tosca Roncada
Verified customer
Submitted Mar 28 2022