Product nameAnti-Aggrecan antibody
See all Aggrecan primary antibodies
DescriptionRabbit polyclonal to Aggrecan
SpecificityThis antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with ab36861.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Dog
- In Western Blot, this antibody gave a positive signal using Aggrecan G1-IGD-G2 Domains recombinant protein It also gave a positive result in FFPE human and rat liver sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab36861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 250 kDa).Can be blocked with Rat Aggrecan peptide (ab36860).
This application has only been tested on recombinant protein.
|ICC/IF||Use at an assay dependent concentration. PubMed: 18462789|
RelevanceAggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
Cellular localizationSecreted; extracellular matrix.
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
Anti-Aggrecan antibody (ab36861) at 1 µg/ml + Aggrecan G1-IGD-G2 recombinant protein
Goat polyclonal to Rabbit IgG H&L (HRP) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 250 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
This antibody was tested on Human Aggrecan G1-IGD-G2 Domains recombinant protein which migrates at approximately 120 kDa.
IHC image of Aggrecan staining in rat liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IHC image of Aggrecan staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36861, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Suna G et al. Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries. Circulation 137:166-183 (2018). Read more (PubMed: 29030347) »
- Lin Y et al. Propionibacterium acnes induces intervertebral disc degeneration by promoting nucleus pulposus cell apoptosis via the TLR2/JNK/mitochondrial-mediated pathway. Emerg Microbes Infect 7:1 (2018). WB . Read more (PubMed: 29323102) »