Anti-Aggrecan antibody - C-terminal (ab140691)
Overview
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Product name
Anti-Aggrecan antibody - C-terminal
See all Aggrecan primary antibodies -
Description
Rabbit polyclonal to Aggrecan - C-terminal -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins, except REG1A (50%). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Hamster, Monkey, Gorilla, Opossum, Common marmoset, Platypus
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Immunogen
16 amino acid synthetic peptide from the C terminal region of Human Aggrecan (NP_037359.2).
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Positive control
- Human cartilage tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
Cellular localization
Secreted; extracellular matrix. -
Database links
- Entrez Gene: 176 Human
- Entrez Gene: 11595 Mouse
- Entrez Gene: 58968 Rat
- Omim: 155760 Human
- SwissProt: P16112 Human
- SwissProt: Q61282 Mouse
- SwissProt: P07897 Rat
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Alternative names
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody - C-terminal (ab140691)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cartilage tissue (after heat induced antigen retrieval) labelling Aggrecan with ab140691 at 10 µg/ml.
References
ab140691 has not yet been referenced specifically in any publications.
