Recombinant Anti-Aggrecan antibody [EPR14664] (ab186414)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-220800-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14664] to Aggrecan
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Aggrecan antibody [EPR14664]
See all Aggrecan primary antibodies -
Description
Rabbit monoclonal [EPR14664] to Aggrecan -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Aggrecan aa 750-900. The exact sequence is proprietary.
Database link: P16112 -
Positive control
- Human cartilage tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14664 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab186414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
Cellular localization
Secreted; extracellular matrix. -
Database links
- Entrez Gene: 176 Human
- Omim: 155760 Human
- SwissProt: P16112 Human
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Alternative names
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)
Immunohistochemical analysis of paraffin embedded Human cartilage tissue sections labeling Aggrecan using ab186414 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-283882-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in normal Human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human colon tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-283884-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in normal Human placenta tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human placenta tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-283883-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in Human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in Human colon carcinoma tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-5-benefits-of-recombinant-antibodies.png)
Anti-Aggrecan antibody [EPR14664] (ab186414)
Datasheets and documents
Certificate of Compliance
References (2)
ab186414 has been referenced in 2 publications.
- Lo Monaco M et al. Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis. Cells 9:N/A (2020). PubMed: 32326610
- Mahboudi H et al. Enhanced chondrogenesis differentiation of human induced pluripotent stem cells by MicroRNA-140 and transforming growth factor beta 3 (TGFß3). Biologicals 52:30-36 (2018). IHC-P . PubMed: 29456027
