Recombinant Anti-Aggrecan antibody [EPR14664] (ab186414)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14664] to Aggrecan
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Aggrecan antibody [EPR14664]
See all Aggrecan primary antibodies -
Description
Rabbit monoclonal [EPR14664] to Aggrecan -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Aggrecan aa 750-900. The exact sequence is proprietary.
Database link: P16112 -
Positive control
- Human cartilage tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14664 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab186414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
Cellular localization
Secreted; extracellular matrix. -
Database links
- Entrez Gene: 176 Human
- Omim: 155760 Human
- SwissProt: P16112 Human
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Alternative names
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-220800-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Immunohistochemical analysis of paraffin embedded Human cartilage tissue sections labeling Aggrecan using ab186414 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-283882-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in normal Human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human colon tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-283884-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in normal Human placenta tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human placenta tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)](https://www.abcam.com/ps/products/186/ab186414/Images/ab186414-283883-anti-aggrecan-antibody-epr14664-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] (ab186414)Ab186414 staining Aggrecan in Human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in Human colon carcinoma tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
Certificate of Compliance
References
ab186414 has been referenced in 1 publication.
- Mahboudi H et al. Enhanced chondrogenesis differentiation of human induced pluripotent stem cells by MicroRNA-140 and transforming growth factor beta 3 (TGFß3). Biologicals 52:30-36 (2018). IHC-P . PubMed: 29456027
