RecombinantRabMAb

Recombinant Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
  • Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14664] to Aggrecan - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Aggrecan antibody [EPR14664] - BSA and Azide free
    See all Aggrecan primary antibodies

  • Description

    Rabbit monoclonal [EPR14664] to Aggrecan - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Aggrecan aa 750-900. The exact sequence is proprietary.
    Database link: P16112

  • Positive control

    • IHC-P: Human cartilage tissue.

  • General notes

    Ab232628 is the carrier-free version of ab186414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232628 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab232628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.

  • Cellular localization

    Secreted; extracellular matrix.

  • Database links

    • Alternative names

      • ACAN antibody
      • AGC 1 antibody
      • AGC1 antibody
      • AGCAN antibody
      • Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
      • Aggrecan 1 antibody
      • Aggrecan core protein antibody
      • Aggrecan proteoglycan antibody
      • Aggrecan structural proteoglycan of cartilage antibody
      • Aggrecan1 antibody
      • ATEGQV antibody
      • Cartilage specific proteoglycan core protein antibody
      • Chondroitin sulfate proteoglycan 1 antibody
      • Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
      • Chondroitin sulfate proteoglycan core protein 1 antibody
      • CSPG 1 antibody
      • CSPG1 antibody
      • CSPGCP antibody
      • JSCATE antibody
      • Large aggregating proteoglycan antibody
      • mcspg antibody
      • mgsk16 antibody
      • MSK 16 antibody
      • MSK16 antibody
      • SEDK antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

      Ab186414 staining Aggrecan in normal Human placenta tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human placenta tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody.  Hematoxylin counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

      Ab186414 staining Aggrecan in Human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in Human colon carcinoma tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody.  Hematoxylin counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

      Ab186414 staining Aggrecan in normal Human colon tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin embedded section). Ab186414 shows negative staining in normal Human colon tissue section. A ready to use HRP Polymer for Rabbit IgG was used as the secondary antibody.  Hematoxylin counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

      Immunohistochemical analysis of paraffin embedded Human cartilage tissue sections labeling Aggrecan using ab186414 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab186414).

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)
      Anti-Aggrecan antibody [EPR14664] - BSA and Azide free (ab232628)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab232628? Please let us know so that we can cite the reference in this datasheet.

    ab232628 has not yet been referenced specifically in any publications.

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