Overview

  • Product name

    Aggresome Detection Kit
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    Abcam's Aggresome Detection kit (ab139486) provides a rapid, specific and quantitative approach to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context. This fixed-cell assay doesn’t require non-physiological protein mutations or genetically engineered cell lines. The Aggresome Detection Reagent has been validated under a wide range of conditions and with small molecule modulators, demonstrating suitability for screening compounds of potential therapeutic value, and optimized for antibody co-localization studies to identify interactions between aggregated protein cargo and the various proteins implicated in autophagy and aggresome formation. Suggested uses for this kit include:

    1. The identification of small molecules that inhibit aggresome formation.

    2. Immuno-localization studies between aggregated protein cargo and the various proteins implicated in aggresome formation, such as histone deacetylase 6, parkin, ataxin-3, dynein motor complex and ubiquilin-1.

     

     

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

Associated products

Images

  • The increase in the fluorescence intensity of the Aggresome Detection Reagent with the addition of MG-132 in Jurkat cells. Jurkat cells were mock induced with 0.08% DMSO (blue line) or induced with 8 μM MG-132 (black line) in a 37ºC, 5% CO2 incubator for 24 hours.

  • Aggresomes were detected in HeLa cells after overnight incubation with a range of proteasome inhibitors, as observed by fluorescence microscopy.

  • Excitation and fluorescence emission spectra for Aggresome reagent dye, Ex/Em=500/600 nm (panel A, left) and Hoechst 33342, Ex/Em=350/461 nm (panel B, right). All spectra were determined in 1X Assay Buffer.

Protocols

References

ab139486 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Testing Drug A with aggresome detection kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Drug A has been tested for aggresome presence in HeLa cells, control cells and MG132-treated cells (5uM for 12h ) have been used as a positive control. The company's instructions were followed for aggresome detection. Imaging was performed on a customized Andor Revolution Spinning Disk Confocal System built around a stand (IX81 Olympus) with a 60x lens and a digital camera (Andor Ixon+885) (CIBIT Facility, MBG-DUTH). Image acquisition was performed in Andor IQ 2 software. Optical sections were recorded every 0.3 µm. All confocal microscopy images presented in this work are 2D maximum intensity projections of z-stack images (ImageJ 1.47v National Institute of Health, USA).
Personal feedback : Due to the photosensitive nature of the aggresome detection dye, special care should be taken with laser power and the exposure time to prevent photobleaching.

Dr. Dimitra Kalamida

Verified customer

Submitted Oct 17 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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