Overview

  • Product name

    Anti-AGK antibody
  • Description

    Rabbit polyclonal to AGK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human AGK aa 1-235.

  • Positive control

    • A431, H1299 cell lysates; Mouse heart; Hep3B cells; H1299 xenograft tissue
  • General notes

    Previously labelled as Acylglycerol Kinase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab137616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
  • Tissue specificity

    Highly expressed in muscle, heart, kidney and brain.
  • Pathway

    Lipid metabolism; glycerolipid metabolism.
  • Sequence similarities

    Contains 1 DAGKc domain.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610037M15Rik antibody
    • 6720408I04Rik antibody
    • Acylglycerol Kinase antibody
    • Acylglycerol kinase mitochondrial antibody
    • Acylglycerol kinase, mitochondrial antibody
    • agk antibody
    • AGK_HUMAN antibody
    • AI465370 antibody
    • FLJ10842 antibody
    • hAGK antibody
    • HsMuLK antibody
    • MuLK antibody
    • Multi substrate lipid kinase antibody
    • Multi-substrate lipid kinase antibody
    • Multiple substrate lipid kinase antibody
    • RGD1562046 antibody
    see all

Images

  • All lanes : Anti-AGK antibody (ab137616) at 1/500 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Anti-AGK antibody (ab137616) at 1/1000 dilution + Mouse heart lysate at 50 µg

    Predicted band size: 47 kDa



    10% SDS-PAGE
  • ab137616 at 1/500 dilution staining AGK in methanol fixed Hep3B cells by Immunofluorescence. Lower image costained with Hoerchst 33342.

  • ab137616 at 1/100 dilution, staining AGK in paraffin embedded H1299 xenograft tissue by Immunohistochemistry.

References

ab137616 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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