Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

Overview

  • Product name

    Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free
    See all AGL/Alpha-glucosidase primary antibodies
  • Description

    Rabbit monoclonal [EPR8880] to AGL/Alpha-glucosidase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide aa 50-150.

  • Positive control

    • Human fetal muscle, mouse muscle, Human fetal heart, K562 and 293T cell lysates, Human liver tissue and Human muscle tissue, HeLa cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab230798 is a PBS-only buffer version of ab133720, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab133720 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

     This product was previously labelled as AGL

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 175 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
  • Tissue specificity

    Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle.
  • Involvement in disease

    Glycogen storage disease 3
  • Sequence similarities

    Belongs to the glycogen debranching enzyme family.
  • Post-translational
    modifications

    The N-terminus is blocked.
    Ubiquitinated.
  • Cellular localization

    Cytoplasm. Under glycogenolytic conditions localizes to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110061O17Rik antibody
    • 4-1 antibody
    • 4-glucantransferase antibody
    • 6-glucosidase antibody
    • 9430004C13Rik antibody
    • 9630046L06Rik antibody
    • AGL antibody
    • AI850929 antibody
    • Amylo 1 6 glucosidase 4 alpha glucanotransferase antibody
    • Amylo-1 antibody
    • Amylo-alpha-1 antibody
    • C77197 antibody
    • Dextrin 6-alpha-D-glucosidase antibody
    • GDE antibody
    • GDE_HUMAN antibody
    • Glycogen debrancher antibody
    • Glycogen debranching enzyme antibody
    • Glycogen storage disease type III antibody
    • Oligo-1 antibody
    see all

Images

  • Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling AGL/Alpha-glucosidase with purified ab133720 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133720).

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling AGL/Alpha-glucosidase with Purified ab133720 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor? 594) 1:200. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling AGL/Alpha-glucosidase with Purified ab133720 at 1:2000 dilution (0.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP)
    secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133720).

  • Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling AGL/Alpha-glucosidase with purified ab133720 at 1/250 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133720).

  • Immunohistochemical analysis of paraffin-embedded Human muscle tissue labelled with unpurified ab133720 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133720).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133720).

  • This IHC data was generated using the same anti-AGL/Alpha-glucosidase antibody clone, EPR8880, in a different buffer formulation (cat# ab133720).

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelled with unpurified ab133720 at 1/100 dilution.

  • This IHC data was generated using the same anti-AGL/Alpha-glucosidase antibody clone, EPR8880, in a different buffer formulation (cat# ab133720).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: AGL/Alpha-glucosidase knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Hek293 whole cell lysate (20 µg
    Lanes 1 - 4: Merged signal (red and green). Green - ab133720 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

    Unpurified ab133720 was shown to specifically react with AGL/Alpha-glucosidase when AGL/Alpha-glucosidase knockout samples were used. Wild-type and AGL/Alpha-glucosidase knockout samples were subjected to SDS-PAGE. 
    Unpurified Ab133720 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

References

ab230798 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230798.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up