Anti-Ago1 antibody (ab129304)
Key features and details
- Goat polyclonal to Ago1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ago1 antibody
See all Ago1 primary antibodies -
Description
Goat polyclonal to Ago1 -
Host species
Goat -
Specificity
ab129304 is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4 -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Turkey, Pig, Xenopus laevis, Pufferfish, Monkey, Zebrafish, Gorilla, Opossum, Common marmoset, Bat, Platypus, Elephant -
Immunogen
Synthetic peptide:
C-KNASYNLDPYIQEF
corresponding to internal sequence amino acids 382-395 of Human Ago1 (NP_036331.1). -
Positive control
- Human Small Intestine and Placenta tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.05% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml.
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Notes |
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IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. |
Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 428222 Chicken
- Entrez Gene: 522972 Cow
- Entrez Gene: 475337 Dog
- Entrez Gene: 100055148 Horse
- Entrez Gene: 26523 Human
- Entrez Gene: 236511 Mouse
- Entrez Gene: 100499510 Pig
- Entrez Gene: 313594 Rat
see all -
Alternative names
- AGO1 antibody
- AGO1_HUMAN antibody
- Argonaute 1 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab129304 has been referenced in 1 publication.
- Luo R et al. POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1. BMC Pulm Med 21:117 (2021). PubMed: 33832481