• Product name
  • Description
    Rabbit polyclonal to Ago1
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Schizosaccharomyces pombe Ago1.

  • Positive control
    • This antibody gave a positive signal in S. Pombe whole cell lysate.



Our Abpromise guarantee covers the use of ab18190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 95 kDa).


  • Function
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
  • Sequence similarities
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
    • Alternative names
      • AGO1 antibody
      • AGO1_HUMAN antibody
      • Argonaute 1 antibody
      • Argonaute 1 RISC catalytic component antibody
      • Argonaute1 antibody
      • eIF 2C 1 antibody
      • eIF-2C 1 antibody
      • eIF2C 1 antibody
      • EIF2C antibody
      • Eif2c1 antibody
      • Eukaryotic translation initiation factor 2C 1 antibody
      • GERP95 antibody
      • Golgi Endoplasmic Reticulum protein 95 kDa antibody
      • hAgo1 antibody
      • Protein argonaute 1 antibody
      • Protein argonaute-1 antibody
      • Putative RNA binding protein Q99 antibody
      • Putative RNA-binding protein Q99 antibody
      • Q99 antibody
      see all


    • Anti-Ago1 antibody (ab18190) at 1 µg/ml + S.pombe Whole Cell Lysate at 10 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size: 95 kDa
      Observed band size: 85 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 16 kDa, 250 kDa, 90 kDa. We are unsure as to the identity of these extra bands.

      Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
    • All lanes : Anti-Ago1 antibody (ab18190) at 1 µg/ml

      Lane 1 : S.pombe wild-type whole cell extract
      Lane 2 : S.pombe ago1Δ whole cell extract
      Lane 3 : S.pombe endogenously expressed tagged Ago1 whole cell extract
      Lane 4 : S.pombe over-expressed tagged Ago1 whole cell extract
      Lane 5 : S.pombe repressed tagged Ago1 whole cell extract

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 95 kDa
      Observed band size: 95 kDa
      Additional bands at: 60 kDa (possible cross reactivity)

      ab18190 faintly recognises Ago1 in the wildtype and expressed tagged Ago1 strains at 95kDa but not in the Ago1Δ or repressed tagged Ago1 as expected. There is a non-specific band at 60kDa, which attributed to cross-reactivity.


    This product has been referenced in:
    • Deng X  et al. Sgf73, a subunit of SAGA complex, is required for the assembly of RITS complex in fission yeast. Sci Rep 5:14707 (2015). ChIP . Read more (PubMed: 26443059) »
    • Shah S  et al. lncRNA recruits RNAi and the exosome to dynamically regulate pho1 expression in response to phosphate levels in fission yeast. Genes Dev 28:231-44 (2014). ChIP ; Schizosaccharomyces pombe . Read more (PubMed: 24493644) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Thank you for your interest in our products.

    In order to be able to help you further, would you be so kind to confirm what issue you have experienced with ab18190 and why you would need a specific protocol for preparing yeast lysates?
    Read More


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